Human IL20RA / IL20R1 ELISA Pair Set
The human IL20RA / IL20R1 ELISA Pair Set is for the quantitative determination of human IL20RA / IL20R1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL20RA /IL20R1 coated on a 96-well plate. Standards and samples are added to the wells,and any IL20RA / IL20R1 present binds to the immobilized antibody. The wellsare washed and a horseradish peroxidase conjugated rabbit anti-IL20RApolyclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of IL20RA / IL20R1 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.