St John's Laboratory
免疫印迹, 酶联免疫吸附测定, 免疫组化-石蜡切片
图像 1 :
Western Blot (WB) analysis of specific lysis using Phospho-Akt1 (T450) Polyclonal Antibody.
Akt1 is a protein encoded by the oncogene AKT1 which is approximately 55,7 kDa. Akt1 is localised to the cytoplasma, nucleus and cell membrane. It is involved in RET signalling, the regulation of lipid metabolism, insulin signalling-generic cascades, common cytokine receptor gamma-chain family signalling pathways and apoptotic pathways. This protein falls under the AKT kinase family. The AKT1 isoform has a specific role in cell motility and proliferation. By activating serine/threonine kinase AKT1 apoptosis can be suppressed by phosphorylating and therby inactivating components of the apoptotic machinery. Mutations in the AKT1 gene can result in breast cancer, colorectal cancer, Cowden syndrome 6 and Proteus syndrome which causes overgrowth. STJ90168 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Akt1 protein which only binds about T450 when T450 is phosphorylated.
人类, 小鼠, 大鼠
AKT1 antibody, PKB antibody, RAC antibody, RAC-alpha serine/threonine-protein kinase antibody, Protein kinase B antibody, PKB antibody, Protein kinase B alpha antibody, PKB alpha antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody, Phospho-Akt1 (T450) antibody
Synthesized peptide derived from human Akt1 around the phosphorylation site of T450.
Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles .
Phospho-Akt1 (T450) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at T450.
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
液体PBS含50% 甘油, 0.5% BSA
免疫印迹, 免疫组化-p, 酶联免疫吸附测定
Store at -20°C, and avoid repeat freeze-thaw cycles.