Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-329
文章摘录数: 7
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Tau (K18) P301L Mutant Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-330
文章摘录数: 5
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Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils: ATTO 488
StressMarq Biosciences
目录: SPR-329-A488
文章摘录数: 6
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Tau-441 (2N4R) Wild-Type Monomers
StressMarq Biosciences
目录: SPR-479
文章摘录数: 4
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Tau Truncated Fragment (AA297-391) (dGAE) Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-461
文章摘录数: 2
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Tau (K18) Delta K280 Mutant Monomers
StressMarq Biosciences
目录: SPR-476
文章摘录数: 2
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Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils (Baculovirus/Sf9)
StressMarq Biosciences
目录: SPR-471
文章摘录数: 3

Thioflavin T assay of tau pre-formed fibrils (PFFs) combined with tau monomers. The PFFs seed the aggregation of the monomers
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Tau-441 (2N4R) Wild-Type Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-480
文章摘录数: 3
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Tau-352 (fetal 0N3R) Wild-Type Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-491
文章摘录数: 2

AFM of Fetal Tau 0N3R fibrils. Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in dH2O, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 10 x 10 µm x-y (left) and 2 x 2 µm x-y (right) both with a z-range of 6 nm. Note: AFM images display significant twisting and curvature not observed under TEM.

Tris-glycine SDS-PAGE (12%) of Fetal Tau 0N3R monomers. MW ladder = Precision Plus Protein All Blue prestained standards.
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Tau (K18) P301L Mutant Monomers
StressMarq Biosciences
目录: SPR-328
文章摘录数: 1
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Tau Truncated Fragment (AA297-391) (dGAE) Monomers
StressMarq Biosciences
目录: SPR-444
文章摘录数: 1

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when truncated tau fragment (AA297-391) (dGAE) monomer is combined with truncated tau fragment (AA297-391) (dGAE) preformed fibrils (Type 1).
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Tau Truncated Fragment (AA297-391) (dGAE C322A) Monomers
StressMarq Biosciences
目录: SPR-445
文章摘录数: 1

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when truncated tau fragment (AA297-391) (dGAE C322A) monomer is combined with truncated tau fragment (AA297-391) (dGAE C322A) preformed fibrils (Type 1).
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Tau Truncated (AA297-391) (dGAE C322A) Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-462
文章摘录数: 1
规格: 100微克
价格:
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Tau-441 (2N4R) P301S Mutant Filaments
StressMarq Biosciences
目录: SPR-463
文章摘录数: 1
规格: 100微克
价格:
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Tau (K18) Delta K280 Mutant Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-477
文章摘录数: 1
规格: 100微克
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Tau-441 (2N4R) P301S Mutant Monomers (Baculovirus/Sf9)
StressMarq Biosciences
目录: SPR-473
文章摘录数: 1

Thioflavin T assay of tau pre-formed fibrils (PFFs) combined with tau monomers. The PFFs seed the aggregation of the monomers
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Tau-441 (2N4R) P301S Mutant Monomers
StressMarq Biosciences
目录: SPR-327
文章摘录数: 1

SDS-PAGE of ~67 kDa Human Tau Protein 2N4R P301S Monomer (SPR-327). Lane 1: MW ladder. Lane 2: Tau Protein Monomer (SPR-327)

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (SPR-327). A greater increase in fluorescence is seen when 50 uM monomer (SPR-327) is combined with 10 nM PFFs (SPR-329 or SPR-330), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau monomers (SPR-327). A greater increase in fluorescence is seen when 50 uM monomer (SPR-327) is combined with 10 nM PFFs (SPR-329 or SPR-330), as the fibrils seed the formation of new fibrils from the pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
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Tau-352 (fetal 0N3R) Wild-Type Monomers
StressMarq Biosciences
目录: SPR-490
文章摘录数: 1

Fibril formation activity of Fetal Tau 0N3R monomers in ThT seeding assay. Monomers readily form fibrils upon the addition of heparin or when seeded by 0N3R pre-formed fibrils. DTT was added to each well to control disulfide bonding during fibril formation.

Fibril formation activity of Fetal Tau 0N3R monomers in ThT seeding assay. Monomers readily form fibrils upon the addition of heparin or when seeded by 0N3R pre-formed fibrils. DTT was added to each well to control disulfide bonding during fibril formation.
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Tau-441 (2N4R) Wild-Type Monomers (Baculovirus/Sf9)
StressMarq Biosciences
目录: SPR-496


SDS-PAGE of purified Sf9/Baculovirus-expressed hTau wild-type 2N4R on a 12% Tris-Glycine Gel. Lane 1: Biorad All Blue Standards (3uL). Lane 2: Baculovirus expressed hTau 2N4R Wild-Type (2ug). Lane 3: Lane 2: Baculovirus expressed hTau 2N4R Wild-Type (4ug).

Dot Blot of purified hTau wild-type 2N4R using phospho-specific Tau antibodies comparing E.coli-expressed material to Sf9/Baculovirus-expressed material. Protein was blotted on nitrocellulose, incubated with 1:1000 primary antibody and/or 1:4000 secondary antibodies. Exposed 0.6s second.

Modified/Total phosphorylation PTM spectrum counts reveal up to 20 phosphorylation sites on human wild-type Tau 2N4R monomers expressed using Baculovirus/Sf9 as determined by mass spectrometry. Protein sequence coverage was 76%. Localization probability cutoff set at ≥80% (yellow) or ≥95% (green).
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Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)
StressMarq Biosciences
目录: SPR-498


TEM of Tau-441 (2N4R) Wild-Type Pre-Formed Fibrils (Baculovirus/Sf9). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 500 nm.

SDS-PAGE of fibrilized Sf9/Baculovirus-expressed hTau wild-type 2N4R on a 12% Tris-Glycine Gel. Lane 1: Biorad All Blue Standards (3uL). Lane 2: Baculovirus expressed hTau 2N4R Wild-Type PFFs (2ug). Lane 3: Baculovirus expressed hTau 2N4R Wild-Type PFFs (4ug).

Modified/Total phosphorylation PTM spectrum counts reveal up to 20 phosphorylation sites on human wild-type Tau 2N4R monomers expressed using Baculovirus/Sf9 as determined by mass spectrometry. Protein sequence coverage was 76%. Localization probability cutoff set at ≥80% (yellow) or ≥95% (green).
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Tau dGAE (297-391) Monomers
StressMarq Biosciences
目录: SPR-501


SDS-PAGE of purified E. coli expressed hTau (dGAE) (SPR-501) on a 12% Tris-Glycine gel.

TEM of Tau dGAE AD-mimic fibrils (SPR-502) generated from Tau dGAE monomers (SPR-501) by shaking 200 rpm at 37oC for 48 hours in 10 mM PB 10 mM DTT pH 7.4 with 200 mM MgCl2 added (Lovestam et al. 2022, eLife). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.

TEM of Tau dGAE AD-mimic fibrils (SPR-502) generated from Tau dGAE monomers (SPR-501) by shaking 200 rpm at 37oC for 48 hours in 10 mM PB 10 mM DTT pH 7.4 with 200 mM MgCl2 added (Lovestam et al. 2022, eLife). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.
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Tau dGAE (297-391) AD-mimic Pre-formed Fibrils
StressMarq Biosciences
目录: SPR-502


TEM of Tau dGAE AD-mimic fibrils (SPR-502) generated from Tau dGAE monomers (SPR-501) by shaking 200 rpm at 37oC for 48 hours in 10 mM PB 10 mM DTT pH 7.4 with 200 mM MgCl2 added (Lovestam et al. 2022, eLife). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 200 nm.

SDS-PAGE of Tau dGAE AD-mimic fibrils (SPR-502) on a 12% Tris-Glycine Gel.

TEM of Tau dGAE AD-mimic fibrils (SPR-502). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 500 nm.
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