ELISA/assay

HO-1酶联免疫吸附试剂盒

SKT-111-96

96 well

524.00美元

HO-1酶联免疫吸附试剂盒

Colorimetric detection of heme oxygenase 1

HO-1

524.00美元

美元

Cancer Oxidative Stress

Heme oxygenase 1 ELISA Kit, Hemox ELISA Kit, HMOX1 ELISA Kit, HO1 ELISA Kit, HO 1 ELISA Kit, HSP32 ELISA Kit

96 well

检测试剂盒

酶联免疫吸附试剂盒

StressXpress®

Colorimetric Assay

Sandwich ELISA (Enzyme-linked Immunosorbent Assay)

Sandwich

Microplate

ELISA kit used to quantitate HO-1 concentration in samples.

1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 50 µL of Pre-Treatment Buffer to all sample and standard wells. 3. Add 50 µL of Standard and sample to appropriate wells. 4. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 2 hours. 5. Wash plate four times with 1X Wash Buffer. 6. Add 100 µL of Biotinylated Antibody Working Solution to each well. 7. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 8. Wash plate four times with 1X Wash Buffer as described in step 5. 9. Add 100 µL of Streptavidin-HRP Working Solution to each well. 10. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 11. Wash plate four times with 1X Wash Buffer as described in step 5. 12. Add 100 µL of TMB Substrate to each well. 13. Develop the plate in the dark at room temperature for 30 minutes. 14. Stop reaction by adding 100 µL of Stop Solution to each well. 15. Measure absorbance on a plate reader at 450 nm.

SKC-111A SKC-111B SKC-111C SKC-111D SKC-111E SKC-111F SKC-111G SKC-111H SKC-111I SKC-111J SKC-111K SKC-111L

Anti-HO-1 Immunoassay Plate 5X HO-1 Extraction Reagent Recombinant HO-1 Standard Standard and Sample Diluent 10X Wash Buffer Concentrate Anti-HO-1 Biotinylated Antibody Concentrate Anti-HO-1 Biotinylated Antibody Diluent Streptavidin: HRP Concentrate Streptavidin: HRP Diluent TMB Substrate Stop Solution Pre-treatment Buffer

1 Plate 1 vial/10 ml 2 vials 1 vial/ 50 ml 1 vial/100 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/150 µl 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml 1 vial/ 13 ml

人类大鼠

Hu Rt

Cell Lysates Tissue Serum Cyst fluid

40 samples in duplicate

30分钟

0.21 ng/ml

0.781 - 50 ng/ml

4ºC

Blue Ice

HO-1 ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-111-96)

Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Kit Booklet MSDS

SKT-111_HO-1_ELISA_Kit_Standard_Curve.png

Graph of the Typical Standard Curve for the HO-1 ELISA Kit StressXpress® - SKT-111