产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
HSP90甲抗体
目录 :
SMC-108D
规格 :
100微克
价格 :
314.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
Hyb-K41009
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
文章摘录数: 7
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类
Xi C, Hu Y, Buckhaults P, Moskophidis D, Mivechi N. Heat shock factor Hsf1 cooperates with ErbB2 (Her2/Neu) protein to promote mammary tumorigenesis and metastasis. J Biol Chem. 2012;287:35646-57 pubmed 出版商
  • 免疫沉淀; 人类; 1:50
Shipp C, Watson K, Jones G. Associations of HSP90 client proteins in human breast cancer. Anticancer Res. 2011;31:2095-101 pubmed
Katoh H, Kubota T, Nakatsu Y, Tahara M, Kidokoro M, Takeda M. Heat Shock Protein 90 Ensures Efficient Mumps Virus Replication by Assisting with Viral Polymerase Complex Formation. J Virol. 2017;91: pubmed 出版商
Koyama S, Narita E, Shimizu Y, Shiina T, Taki M, Shinohara N, et al. Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells. Int J Environ Res Public Health. 2016;13: pubmed 出版商
Koyama S, Narita E, Shimizu Y, Suzuki Y, Shiina T, Taki M, et al. Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye. Int J Environ Res Public Health. 2016;13: pubmed 出版商
Ishikawa Y, Sakurai H. Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells. FEBS J. 2015;282:332-40 pubmed 出版商
Quanz M, Herbette A, Sayarath M, de Koning L, Dubois T, Sun J, et al. Heat shock protein 90? (Hsp90?) is phosphorylated in response to DNA damage and accumulates in repair foci. J Biol Chem. 2012;287:8803-15 pubmed 出版商
产品信息
目录号 :
SMC-108D
产品名称 :
HSP90甲抗体
描述 :
小鼠抗人类HSP90甲单克隆IgG2a
靶标 :
HSP90甲
共轭标签 :
未共轭
2021标价 :
314.00美元
Currency :
美元
研究领域(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers
别称(s) :
HSP86抗体, HSP89A抗体, HSP90A抗体, HSP90AA1抗体, HSP90Alpha抗体, HSPC1抗体, HSPCA抗体, HSPCAL3抗体
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
Hyb-K41009
免疫原 :
Recombinant human HSP90alpha; Specificity mapped to amino acids 604-731
免疫原物种 :
人类
登录号 :
NP_001017963.2
Swiss Prot数据库 :
P07900
应用 :
免疫印迹免疫组化酶联免疫吸附测定
宿主物种 :
小鼠
抗体亚型 :
IgG2a
物种活性缩写 :
Hu Ms Rt
物种活性全称 :
人类小鼠大鼠
抗体稀释 :
WB (1:1000), IHC (1:5000); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.2, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects 90kDa. This is an alpha-specific product.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-108D, RRID: AB_854218
化验分析证明书 :
1 µg/ml of SMC-108 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
细胞质黑素体
科研背景 :
HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90, are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite it's label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
参考文献 :
1. Nemoto, T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami, Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem. 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem. 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med. 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med. 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev. 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med. 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA. 91: 8324–8328. 10. Nemoto, T. (1997) Biochem and Mol. Bio Intl. 42 (5): 881-889.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-108_Hsp90-alpha_Antibody_K41009_IHC
_Mouse_inflamed-colon_40x_1.png
SMC-108_Hsp90-alpha_Antibody_K41009_IHC_Human_colon-carcinoma_40x_1.png SMC-108_Hsp90-alpha_Antibody_K41009_WB_Rat_Lysates_1.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。