抗体

HSP60抗体

SMC-111B

200微克

276.00美元

单克隆

小鼠

未共轭

LK2

人类, 小鼠, 大鼠, 犬, 牛

免疫印迹, 免疫组化, 流式细胞仪

HSP60抗体

小鼠抗人类HSP60单克隆IgG1

HSP60

未共轭

276.00美元

美元

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Tags and Cell Markers Organelle Markers

CPN60抗体, GROEL抗体, HLD4抗体, HSP 60抗体, HSP65抗体, HSPD1抗体, HuCHA60抗体, SPG 13抗体

200微克

抗体

单克隆

LK2

重组人类HSP60

人类

NP_002147.2

P10809

免疫印迹免疫组化FCM

小鼠

IgG1

Hu Ms Rt Bv Dg Ck GP Hm Mk Pg Rb Pl Ba T.sp Ys Is Fs

Human Mouse Rat Bovine Dog Chicken Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit Plant Spinach Bacteria E. coli (Escherichia coli) H. pylori (Helicobacter pylori) Bacteria (Salmonella Typhimurium) Nematode (Trichinella spiralis) Yeast Insect White Fly (Aleyrodidae) Fish Three-spined stickleback (Gasterosteus aculeatus)

WB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.

蛋白质G纯化

PBS, 50% 甘油, 0.1mM苯甲基磺酰氟

1毫克/毫升

Detects ~60kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-111B, RRID: AB_2121271

0.25 µg/ml of SMC-111 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.

Mitochondrion Mitochondrion Matrix

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).

1. Hartl, F.U. (1996) Nature 381: 571-579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366. 3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858. 4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. 10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556. 11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

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SMC-111_HSP60_Antibody_LK2_IHC_Human_Ki
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