抗体

CaMKII (Alpha-Specific) Antibody

SMC-124D

100微克

268.00美元

单克隆

小鼠

未共轭

6G9

人类, 小鼠, 大鼠, 牛

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 放射免疫分析法

CaMKII (Alpha-Specific) Antibody

Mouse Anti-Rat CaMKII (Alpha-Specific) Monoclonal IgG1

CaMKII

未共轭

268.00美元

美元

Cell Signaling Post-translational Modifications Phosphorylation

CamK2抗体, CamK2A抗体, CamK2B抗体, CamK2D抗体, CamK2G抗体, CAMKA抗体

100微克

抗体

单克隆

6G9

Partially purified rat CaMKII

大鼠

NP_033922.1

P11798

免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定RIA

小鼠

IgG1

Hu Ms Rt Bv

人类小鼠大鼠牛

WB (1:10000), IHC (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.

蛋白质G纯化

PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠

1毫克/毫升

Detects ~50-60kDa. Recognizes both phosphorylated and non-phosphorylated forms.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-124D, RRID: AB_2275062

0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Cytoplasm Mitochondrion Nucleus Cell Junction Synapse Presynaptic Cell Membrane

CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).

1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

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