产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
CaMKII抗体
目录 :
SMC-125D
规格 :
100微克
价格 :
278.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
22B1
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
文章摘录数: 1
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:500
Feng T, Szabo E, Dziak E, Opas M. Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells. Stem Cell Rev. 2010;6:74-85 pubmed 出版商
产品信息
目录号 :
SMC-125D
产品名称 :
CaMKII抗体
描述 :
小鼠抗大鼠CaMKII单克隆IgG1
靶标 :
CaMKII
共轭标签 :
未共轭
2021标价 :
278.00美元
Currency :
美元
研究领域(s) :
Cell Signaling Post-translational Modifications Phosphorylation
别称(s) :
csaid结合蛋白1抗体, CSBP1抗体, CSBP2抗体, EXIP抗体, 地图激酶MXI2抗体, MAPkinase p38alpha抗体, MAPK14抗体, p38甲抗体, p38 MAP激酶抗体, p丝裂原活化蛋白激酶抗体, RK抗体, SAPK 2A抗体, 应激活化蛋白激酶2a抗体
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
22B1
免疫原 :
合成肽
免疫原物种 :
大鼠
登录号 :
NP_037052.1
Swiss Prot数据库 :
P11275
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定
宿主物种 :
小鼠
抗体亚型 :
IgG1
物种活性缩写 :
Hu Ms Rt
物种活性全称 :
人类小鼠大鼠
抗体稀释 :
WB (1:1000), IHC (1:100), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-125D, RRID: AB_2067924
化验分析证明书 :
1 µg/ml was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cellular Localization :
Cytoplasm Mitochondrion Nucleus Cell Junction Synapse Presynaptic Cell Membrane
科研背景 :
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
参考文献 :
1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-125_CaMKII_Antibody_22B1_IHC_Human_colon-carcinoma_40x_1.png SMC-125_CaMKII_Antibody_22B1_WB_Mouse_Ventricle-lysates_1.png
SMC-125_CaMKII_Antibody_22B1_IHC_Mouse_
backskin_8.png
SMC-125_CaMKII_Antibody_22B1_ICC-IF_Rat_dissociated-hippocampal-neurons_10X_1.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。