产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
HO-1抗体
目录 :
SMC-131D
规格 :
100微克
价格 :
304.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
1F12-A6
反应物种 :
人类, 小鼠, 大鼠, 犬, 牛
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
文章摘录数: 5
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠
Al Rifai N, Rücker H, Amslinger S. Opening or closing the lock? When reactivity is the key to biological activity. Chemistry. 2013;19:15384-95 pubmed 出版商
Wang H, Zhang Y, Xia F, Zhang W, Chen P, Yang G. Protective effect of silencing Stat1 on high glucose-induced podocytes injury via Forkhead transcription factor O1-regulated the oxidative stress response. BMC Mol Cell Biol. 2019;20:27 pubmed 出版商
Yamaguchi Y, Kanzaki H, Katsumata Y, Itohiya K, Fukaya S, Miyamoto Y, et al. Dimethyl fumarate inhibits osteoclasts via attenuation of reactive oxygen species signalling by augmented antioxidation. J Cell Mol Med. 2018;22:1138-1147 pubmed 出版商
Kanzaki H, Shinohara F, Itohiya Kasuya K, Ishikawa M, Nakamura Y. Nrf2 activation attenuates both orthodontic tooth movement and relapse. J Dent Res. 2015;94:787-94 pubmed 出版商
Kanzaki H, Shinohara F, Kajiya M, Fukaya S, Miyamoto Y, Nakamura Y. Nuclear Nrf2 induction by protein transduction attenuates osteoclastogenesis. Free Radic Biol Med. 2014;77:239-48 pubmed 出版商
产品信息
目录号 :
SMC-131D
产品名称 :
HO-1抗体
描述 :
Mouse Anti-Human HO-1 Monoclonal IgG1 Kappa
靶标 :
HO-1
共轭标签 :
未共轭
2021标价 :
304.00美元
Currency :
美元
研究领域(s) :
Cancer Oxidative Stress Neuroscience Neurodegeneration Alzheimer's Disease Cardiovascular System Blood Platelets Cell Signaling Epigenetics and Nuclear Signaling Nuclear Signaling Pathways NFkB Pathway Cardiovascular System Atherosclerosis Vascular Inflammation Inflammatory Mediators Cardiovascular System Vasculature Endothelium Cancer Cancer Metabolism Response to Hypoxia Cell Signaling Metabolism Metabolism processes Hypoxia
别称(s) :
HSP32 Antibody, HMOX1 Antibody, Heme oxygenase 1 Antibody, HO Antibody, HO1 Antibody, , 32 kD antibody, bK286B10 antibody, D8Wsu38e antibody, heat shock protein 32 kD antibody, heat shock protein 32kD antibody, Heat shock protein antibody, Heme oxygenase (decycling) 1 antibody, Heme oxygenase 1 antibody, Hemox antibody, HMOX 1 antibody, Hmox antibody, Hmox1 antibody, HMOX1_HUMAN antibody, HO 1 antibody, HO antibody, HO-1 antibody, HO1 antibody, Hsp32 antibody
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
1F12-A6
免疫原 :
人类HO-1合成肽, 氨基酸1-30
免疫原物种 :
人类
登录号 :
NP_002124.1
Swiss Prot数据库 :
P09601
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定
宿主物种 :
小鼠
抗体亚型 :
IgG1 Kappa
物种活性缩写 :
Hu Ms Rt Bv Dg GP Hm Mk Pg Rb
物种活性全称 :
Human Mouse Rat Bovine Dog Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit
抗体稀释 :
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects 32kDa. Does not cross-react with HO-2.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-131D, RRID: AB_2264116
化验分析证明书 :
1 µg/ml was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Cellular Localization :
微粒体内质网
科研背景 :
Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).
参考文献 :
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-131_HO-1_Antibody_1F12-A6_ICC-IF_Human_HeLa-Cells_100x_Composite.png SMC-131_HO-1_Antibody_1F12-A6_IHC_Mouse_backskin_6.png SMC-131_HO-1_Antibody_1F12-A6_ICC-IF_Human_HeLa-Cells_20x_Composite.png SMC-131_HO-1_Antibody_1F12-A6_WB_Human_HeLa-cell-lysates_1.png SMC-131_HO-1_Antibody_1F12-A6_ICC-IF_Human_HaCaT-cells_2.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。