抗体

HSP90(总数)抗体

SMC-149B

200微克

314.00美元

单克隆

小鼠

未共轭

4F3.E8

人类, 小鼠, 大鼠, Canadian hard winter wheat

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀

HSP90(总数)抗体

小鼠抗人类HSP90(总数)单克隆IgG2a

HSP90

未共轭

314.00美元

美元

Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers

HSP84抗体, HSP86抗体, HSP90A抗体, HSP90AA1抗体, HSP90AB1抗体, HSP90B抗体, HSPC1抗体, HSPC2抗体, HSPCAL1抗体, HSPCAL4抗体

200微克

抗体

单克隆

4F3.E8

重组人类HSP90纯化来自大肠杆菌

人类

NP_001017963.2

P07900

免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定

小鼠

IgG2a

Hu Ms Rt Pl

Human Mouse Rat Plant Wheat (Triticum spp.)

WB (1:2000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

蛋白质G纯化

PBS pH7.2, 50% 甘油, 0.09% 叠氮化钠

1毫克/毫升

Detects ~90kDa. This antibody detects both α and β forms of HSP90 equally well.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SMC-149B, RRID: AB_2233312

0.5 µg/ml of SMC-149 was sufficient for detection of HSP90alpha in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

细胞质黑素体

HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85% sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species; having 60% and 78% amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.

1. Nemoto T. et al. (1997) J.Biol Chem. 272: 26179-26187. 2. Minami, Y, et al. (1991), J.Biol Chem. 266: 10099-10103. 3. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 4. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 5. Neckers L, et al. (2002) Trends Mol Med 8: S55-S61. 6. Pratt W, Toft D. (2003) Exp Biol Med 228: 111-133. 7. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 8. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 9. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 10. Nemoto, T. (1997) Biochem and Mol. Bio Intl. 42 (5): 881-889.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SMC-149_Hsp90_Antibody_4F3-E8_ICC-IF_Human_HaCaT-cells_1.png SMC-149_Hsp90_Antibody_4F3-E8_IHC_Human_colon-carcinoma_40x_1.png SMC-149_Hsp90_Antibody_4F3-E8_WB_Rat_tissue-lysate_1.png SMC-149_Hsp90_Antibody_4F3-E8_IHC_Mouse_muscle-tissue_2.png SMC-149_Hsp90_Antibody_4F3-E8_IHC_Mouse_backskin_8.png