产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
DNA/RNA Damage Antibody
目录 :
SMC-155D
规格 :
100微克
价格 :
278.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
15A3
反应物种 :
人类
应用 :
酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类
Aboud O, Mrak R, Boop F, Griffin W. Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype. Acta Neuropathol Commun. 2013;1:41 pubmed 出版商
Wu P, Scarlata E, O Flaherty C. Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa. Antioxidants (Basel). 2020;9: pubmed 出版商
Angeloni C, Teti G, Barbalace M, Malaguti M, Falconi M, Hrelia S. 17β-Estradiol enhances sulforaphane cardioprotection against oxidative stress. J Nutr Biochem. 2017;42:26-36 pubmed 出版商
Liu Y, O Flaherty C. In vivo oxidative stress alters thiol redox status of peroxiredoxin 1 and 6 and impairs rat sperm quality. Asian J Androl. 2017;19:73-79 pubmed 出版商
Lood C, Blanco L, Purmalek M, Carmona Rivera C, De Ravin S, Smith C, et al. Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nat Med. 2016;22:146-53 pubmed 出版商
Angeloni C, Malaguti M, Rizzo B, Barbalace M, Fabbri D, Hrelia S. Neuroprotective effect of sulforaphane against methylglyoxal cytotoxicity. Chem Res Toxicol. 2015;28:1234-45 pubmed 出版商
Ozkosem B, Feinstein S, Fisher A, O Flaherty C. Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice. Redox Biol. 2015;5:15-23 pubmed 出版商
Zawada W, Mrak R, Biedermann J, Palmer Q, Gentleman S, Aboud O, et al. Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related nucleic acid oxidation and caspase-3 activation. Acta Neuropathol Commun. 2015;3:9 pubmed 出版商
Brennan L, Haukedal J, Earle J, Keddie B, Harris H. Disruption of redox homeostasis leads to oxidative DNA damage in spermatocytes of Wolbachia-infected Drosophila simulans. Insect Mol Biol. 2012;21:510-20 pubmed 出版商
产品信息
目录号 :
SMC-155D
产品名称 :
DNA/RNA Damage Antibody
描述 :
Mouse Anti- DNA/RNA Damage Monoclonal IgG2b
靶标 :
DNA损伤
共轭标签 :
未共轭
2021标价 :
278.00美元
Currency :
美元
研究领域(s) :
Cancer Oxidative Stress Cell Signaling Post-translational Modifications Oxidation Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Damage and Repair
别称(s) :
8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, 8OHG Antibody, 80G Antibody, 8 hydroxyguanine Antibody, 8 hydroxy 2' deoxyguanosine Antibody, 8 hydroxyguanosine Antibody, 8 OHG Antibody, 8-OHG Antibody, 8OHdG Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
Trademark :
MOLECULAR SIGNATURE®
克隆编号 :
15A3
免疫原 :
8-hydroxy-guanosine-BSA and –casein conjugates
应用 :
免疫组化免疫细胞化学/IF酶联免疫吸附测定DB免疫沉淀FCM FA
宿主物种 :
小鼠
抗体亚型 :
IgG2b
物种活性缩写 :
都是
物种活性全称 :
Species Independent
抗体稀释 :
IHC (1:1000); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-155D, RRID: AB_889490
科研背景 :
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).
参考文献 :
1. Kim H.W., Murakami A., Williams M.V., and Ohigashi H. (2003) Carcinogenesis 24(2): 235-241. 2. Pilger A. and Rudiger H.W. (2006) Int Arch Occup Environ Health. 80(1): 1-15. 3. Malins D.C. and Haimanot R. (1991) Cancer Res. 51(19): 5430-5432. 4. Kvam E. and Tyrrell R.M. (1997) Carcinogenesis 18(11): 2281-2283. 5. Kowluru R.A., Atasi L., and Ho Y.S. (2006) Invest Ophthalmol Vis Sci 47(4): 1594-9. 6. Bowers R. et al. (2004) Am J Respir Crit Care Med. 169(6): 764-9. 7. Cui J., Holmes E.H., Greene T.G., and Liu P.K. (2000) Faseb J. 14(7): 955-67.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-155_DNA-Damage_Antibody_15A3_IHC_Rat_ischemic-fresh-brain-tissue_1.png
SMC-155_DNA-Damage_Antibody_15A3_IHC_Mo
use_backskin_1.png
SMC-155_DNA-Damage_Antibody_15A3_IHC_Mouse_Retinal-Injury-Model_1.png SMC-155_DNA-Damage_Antibody_15A3_IHC_Mouse_inflamed-colon_40x_1.png
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。