产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
HSP27抗体
目录 :
SMC-161B
规格 :
200微克
价格 :
251.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
5D12-A12
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
文章摘录数: 6
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:1500; 图 15
  • 免疫印迹; 人类; 1:1500; 图 13a
Sugiyama S, Yoshino Y, Kuriyama S, Inoue M, Komine K, Otsuka K, et al. A Curcumin Analog, GO-Y078, Effectively Inhibits Angiogenesis through Actin Disorganization. Anticancer Agents Med Chem. 2016;16:633-47 pubmed
  • 免疫组化; 人类
Kötter S, Unger A, Hamdani N, Lang P, Vorgerd M, Nagel Steger L, et al. Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins. J Cell Biol. 2014;204:187-202 pubmed 出版商
Pereira M, Tomé D, Domingues A, Varanda A, Paulo C, Santos M, et al. A Fluorescence-Based Sensor Assay that Monitors General Protein Aggregation in Human Cells. Biotechnol J. 2018;13:e1700676 pubmed 出版商
Unger A, Beckendorf L, Böhme P, Kley R, von Frieling Salewsky M, Lochmuller H, et al. Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere function. Acta Neuropathol Commun. 2017;5:72 pubmed 出版商
Lee S, Dempsey Hibbert N, Vimalachandran D, Wardle T, Sutton P, Williams J. Re-examining HSPC1 inhibitors. Cell Stress Chaperones. 2017;22:293-306 pubmed 出版商
Mymrikov E, Daake M, Richter B, Haslbeck M, Buchner J. The Chaperone Activity and Substrate Spectrum of Human Small Heat Shock Proteins. J Biol Chem. 2017;292:672-684 pubmed 出版商
产品信息
目录号 :
SMC-161B
产品名称 :
HSP27抗体
描述 :
Mouse Anti-Human HSP27 Monoclonal IgG2b Kappa
靶标 :
HSP27
共轭标签 :
未共轭
2021标价 :
251.00美元
Currency :
美元
研究领域(s) :
Cell Signaling Protein Trafficking Chaperone Proteins Cell Signaling Cytoskeleton Microfilaments Actin Actin Assembly Cell Signaling Cytoskeleton Microtubules Cancer Tumor Biomarkers Cardiovascular System Atherosclerosis Cardiovascular System Heart Contractility Cancer Heat Shock
别称(s) :
28kDa热休克蛋白抗体, CMT2F抗体, HSP25抗体, HSP27抗体, HSP28抗体, HSPB1抗体, SRP27抗体
规格 :
200微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
5D12-A12
免疫原 :
全长人类HSP27
免疫原物种 :
人类
登录号 :
NP_001531.1
Swiss Prot数据库 :
P04792
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定
宿主物种 :
小鼠
抗体亚型 :
IgG2b Kappa
物种活性缩写 :
Hu
物种活性全称 :
人类
抗体稀释 :
WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~27kDa. Has no cross-reactivity to Alpha B crystallin. Very limited cross-reactivity to other species.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-161B, RRID: AB_2248586
化验分析证明书 :
0.5 µg/ml of SMC-161 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus Cytoskeleton Spindle
科研背景 :
HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.
参考文献 :
1. Kim K.K., Kim R., and Kim, S. (1998) Nature 394(6693): 595-599. 2. Van Montfort R., Slingsby C., and Vierling E. (2001) Addv Protein Chem. 59: 105-56. 3. Ehrnsperger M., Graber S., Gaestel M. and Buchner J. (1997) EMBO J. 16: 221-229. 4. Ciocca D.R., Oesterreich S., Chamness G.C., McGuire W.L., and Fugua S.A. (1993) J Natl Cancer Inst. 85 (19): 1558-70. 5. Sarto C., Binnz P.A., and Mocarelli P. (2000) Electrophoresis. 21(6): 1218-26. 6. Arrigo A.P. (2005) J Cell Biochem. 94(2): 241-6.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-161_Hsp27_Antibody_5D12-A3_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SMC-161_Hsp27_Antibody_5D12-A3_ICC-IF_Human_HaCaT-cells_2.png SMC-161_Hsp27_Antibody_5D12-A3_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SMC-161_Hsp27_Antibody_5D12-A3_WB_Human_Cell-lysates_1.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。