产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
磷酸酪氨酸抗体
目录 :
SMC-174D
规格 :
100微克
价格 :
289.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
G104
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
产品信息
目录号 :
SMC-174D
产品名称 :
磷酸酪氨酸抗体
描述 :
小鼠抗磷酸酪氨酸单克隆IgG1
靶标 :
磷酸酪氨酸
共轭标签 :
未共轭
2021标价 :
289.00美元
Currency :
美元
研究领域(s) :
Cell Signaling Post-translational Modifications Phosphorylation
别称(s) :
PhosphoTyrosine (pY) Antibody, PhosphoTyrosine (pY) Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
G104
免疫原 :
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀
宿主物种 :
小鼠
抗体亚型 :
IgG1
物种活性缩写 :
都是
物种活性全称 :
Species Independent
抗体稀释 :
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-174D, RRID: AB_2034404
化验分析证明书 :
1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
科研背景 :
Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
参考文献 :
1. Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11): 6408-6418. 2. Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6): 3811-3818. 3. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 4. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 5. Downward J. (2001) Nature 411: 759-762. 6. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 7. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 8. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 9. Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem. 274(39): 27768-27775.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-174_Phosphotyrosine_Antibody_G104_WB_Human_A431-cell-lysates_1.png
SMC-174_Phosphotyrosine_Antibody_G104_I
HC_Mouse_backskin_2.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。