产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
DMPO抗体
目录 :
SMC-189D
规格 :
100微克
价格 :
310.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
N1664A
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
产品信息
目录号 :
SMC-189D
产品名称 :
DMPO抗体
描述 :
小鼠抗DMPO单克隆IgG1
靶标 :
DMPO
共轭标签 :
未共轭
2021标价 :
310.00美元
Currency :
美元
研究领域(s) :
Cancer Oxidative Stress Cell Signaling Cell Signaling Metabolism Mitochondrial Metabolism Mitochondrial Markers Cell Signaling Metabolism Redox Metabolism
别称(s) :
5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide Antibody, DMPO nitrone adduct Antibody, 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
N1664A
免疫原 :
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定艾姆斯氏试验
宿主物种 :
小鼠
抗体亚型 :
IgG1
物种活性缩写 :
都是
物种活性全称 :
Species Independent
抗体稀释 :
WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross react with non-adducted proteins or DNA.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-189D, RRID: AB_10703686
化验分析证明书 :
A 1:1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
科研背景 :
The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states (1). The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals (1). DMPO adducts can be generated with protein and DNA radicals (7).
参考文献 :
1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141. 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522. 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473–1481. 4. Haseloff R.F., et al. (1997) FEBS Lett 418:73–75. 5. Schaefer C.F., Janzen E.G., West M.S., Poyer J.L., and Kosanke S.D. (1996) Free Radic. Biol. Med 21:427–436. 6. Anzai K., et al. (2003) Arch. Biochem. Biophys 415:251–256. 7. Free Radic Biol Med. (2009) April 1; 46(7): 853–865. doi:10.1016/j.freeradbiomed.2008.12.020. 8. Chatterjee S., et al. (2009) Free Radic. Med.and Biol. 46: 454-461.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-189_DMPO_Antibody_N1664A_ICC-IF_Mouse_macrophage-cell-line-Raw-264.7_1.png SMC-189_DMPO_Antibody_N1664A_WB_Human_HL-60-clone-15-eosinophils-lysates_1.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。