产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
DNMT1抗体
目录 :
SMC-200D
规格 :
100微克
价格 :
300.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
60B1220.1
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫沉淀, 染色质免疫沉淀
更多信息或购买 :
StressMarq Biosciences 产品网页
产品信息
目录号 :
SMC-200D
产品名称 :
DNMT1抗体
描述 :
Mouse Anti-Human DNMT1 Monoclonal IgG1 Kappa
靶标 :
DNMT1
共轭标签 :
未共轭
2021标价 :
300.00美元
Currency :
美元
研究领域(s) :
Cell Signaling Post-translational Modifications Organelle Markers
别称(s) :
DNA Mtase Antibody, DNMT Antibody, MCMT Antibody, DNA methyltransferase 1 Antibody, AIM Antibody, CXXC9 Antibody, DNMT Antibody, DNA (cytosine-5)-methyltransferase 1 Antibody, CXXC-type zinc finger protein 9 Antibody, DNA methyltransferase HsaI Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
60B1220.1
免疫原 :
抗a合成多肽对应于氨基酸637-650人的DNMT1
免疫原物种 :
人类
登录号 :
NP_001370
Swiss Prot数据库 :
P26358
应用 :
免疫印迹免疫组化免疫沉淀ChIP
宿主物种 :
小鼠
抗体亚型 :
IgG1 Kappa
物种活性缩写 :
Hu Ms Fs Zf
物种活性全称 :
Human Mouse Fish Zebrafish (Danio rerio)
抗体稀释 :
WB (1:1000), IHC (1:1000); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~180kDa. It will cross-react with mouse DNMT1.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-200D, RRID: AB_2293057
化验分析证明书 :
2 µg/ml of SMC-200 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus
科研背景 :
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
参考文献 :
1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D., et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S., et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3): 338-342.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-200_DNMT1_Antibody_60B1220-1_IHC_Human_colon-carcinoma_40x_1.png SMC-200_DNMT1_Antibody_60B1220-1_WB_Human_H1299-cell-lysate_2.png SMC-200_DNMT1_Antibody_60B1220-1_IHC_Mouse_medullar-kidney-tissue_1.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。