产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
CD45抗体
目录 :
SMC-256D
规格 :
100微升
价格 :
260.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
12A9
反应物种 :
人类
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
StressMarq Biosciences 产品网页
图像
图像 1 :
StressMarq Biosciences SMC-256D 图像 1
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~147 kDa CD45 protein using Mouse Anti-CD45 Monoclonal Antibody, Clone 12A9 (SMC-256). Primary Antibody: Mouse Anti-CD45 Monoclonal Antibody (SMC-256) at 1:1000. Predicted/Observed Size: ~147 kDa.
产品信息
目录号 :
SMC-256D
产品名称 :
CD45抗体
描述 :
小鼠抗人类CD45单克隆IgG1
靶标 :
CD45
共轭标签 :
未共轭
2021标价 :
260.00美元
Currency :
美元
研究领域(s) :
Immunology Adaptive Immunity B Cells B Cell CD Markers Immunology Adaptive Immunity T Cells T Cell CD Markers Immunology Cell Type Markers CD Markers Non-Lineage Markers Cancer Cancer Growth Inhibitors Tyrosine Kinase Inhibitors Neuroscience Cell Markers Glial Markers Microglia Markers Stem Cells Hematopoietic Progenitors HP Surface Molecules B Lymphocytic Lineage T Lymphocytic Lineage NK Cell Lineage Stem Cells Hematopoietic Progenitors Myeloid Dendritic Cell Lineage Lymphoid Monocytic Lineage Neutrophil Lineage Myeloid Mast Cell Lineage Immunology Adaptive Immunity Regulatory T Cells Angiogenesis
别称(s) :
B220 Antibody, CD 45 Antibody, CD45R Antibody, GP180 Antibody, LCA Antibody, Protein tyrosine phosphatase receptor type c polypeptide Antibody, PTPRC_HUMAN Antibody, T200 glycoprotein Antibody
规格 :
100微升
类别 :
抗体
产品类型 :
单克隆
克隆编号 :
12A9
免疫原 :
纯化重组片段对应于人CD45在大肠杆菌中表达
免疫原物种 :
人类
登录号 :
NP_563578.2
Swiss Prot数据库 :
P08575
应用 :
免疫印迹免疫组化
宿主物种 :
小鼠
抗体亚型 :
IgG1
物种活性缩写 :
Hu
物种活性全称 :
人类
抗体稀释 :
WB (1:1000); optimal dilutions for assays should be determined by the user.
储存缓冲液 :
PBS (without Mg2+ and Ca2+), 50% glycerol, pH 7.4, 150mM NaCl, 0.02% sodium azide
特异性 :
Detects endogenous CD45 proteins. (~147KD).
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SMC-256D, RRID: AB_2699754
化验分析证明书 :
A 1:1000 dilution of SMC-256 was sufficient for detection of CD45 in 10 µg of Human HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Cellular Localization :
膜
科研背景 :
CD45 is a 180-240 kD glycoprotein, also known as the leukocyte common antigen (LCA), T200 or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, broadly expressed amount hematopoietic cells. There are kmultiple isoforms of CD45 distributed throughout the immune system, and they arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell, as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
参考文献 :
1. Barclay A., et al. (1997) The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge I.S., et al. (1993) Annu. Rev. Immunol. 12:85. 3. Kishihara K., et al. (1993) Cell. 74:143. 4. Pulido R., et al. (1988) J. Immunol. 140:3851.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SMC-256_CD45_Antibody_WB_HeLa_1.png
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。