产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
HSP70抗体
目录 :
SPC-103D
规格 :
100微升
价格 :
297.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 3
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 6a
Watanabe Y, Tsujimura A, Taguchi K, Tanaka M. HSF1 stress response pathway regulates autophagy receptor SQSTM1/p62-associated proteostasis. Autophagy. 2017;13:133-148 pubmed 出版商
Woodford M, Dunn D, Blanden A, Capriotti D, Loiselle D, Prodromou C, et al. The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding. Nat Commun. 2016;7:12037 pubmed 出版商
Codonho B, Costa S, Peloso E, Joazeiro P, Gadelha F, Giorgio S. HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics. Mem Inst Oswaldo Cruz. 2016;: pubmed 出版商
产品信息
目录号 :
SPC-103D
产品名称 :
HSP70抗体
描述 :
兔抗人类HSP70多克隆
靶标 :
HSP70
共轭标签 :
未共轭
2021标价 :
297.00美元
Currency :
美元
研究领域(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers
别称(s) :
HSP70 1抗体, HSP70 2抗体, HSP70.1抗体, HSP72抗体, HSP73抗体, HSPA1抗体, HSPA1A抗体, HSPA1B抗体
规格 :
100微升
类别 :
抗体
产品类型 :
多克隆
免疫原 :
全长蛋白HSP70
免疫原物种 :
人类
登录号 :
NP_005336.3
Swiss Prot数据库 :
P0DMV8/P0DMV9
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定
宿主物种 :
物种活性缩写 :
Hu Ms Rt Bg Bv Dg Fs Cp GP Hm Mk Pg Sh Co Pl Shk L. am
物种活性全称 :
Human Mouse Rat Beluga (Delphinapterus leucas) Bovine Dog Fish Carp (Cypriniformes) Guinea Pig (Cavia porcellus) Hamster Monkey Pig Sheep Coral (Anthozoa) Plant Tomato Tobacco Shark Spiny Dogfish Shark (Squalus acanthias) Atlantic Hagfish (Myxine glutinosa) Leishmania amazonensis
抗体稀释 :
WB (1:1000), IHC (1:100), ICC/IF (1:100), IP (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-103D, RRID: AB_2119380
化验分析证明书 :
A 1:1000 dilution of SPC-103 was sufficient for detection of HSP70 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization :
细胞质
科研背景 :
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1, 2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATPbinding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
参考文献 :
1. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050. 2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294. 6. Fink A.L. (1999) Physiol. Rev. 79: 425-449. 7. Hung T.H., et al. (2001) Am J Pathol. 159: 1031-1043. 8. Locke M. (2000) Cell Stress & Chaperones 5: 45-51. 9. Ianaro A., et al. (2001) FEBS Lett. 508: 61-66. 10.Trentin G.A. et al. (2001) J Biol Chem. 276: 13087-13095.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-103_Hsp70_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-103_HSP70_Antibody_IHC_Human_colon-carcinoma_1.png SPC-103_HSP70_Antibody_WB_Human_Rat_brain-cell-lysates_1.png SPC-103_HSP70_Antibody_IHC_Mouse_backskin_3.png SPC-103_HSP70_Antibody_WB_Mouse_Pam212-cells_1.png SPC-103_Hsp70_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SPC-103_HSP70_Antibody_IHC_Mouse_Inflamed-colon_1.png
Image Alternative Text :
Rabbit Anti-Hsp70 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-103) Rabbit Anti-HSP70 Antibody used in Immunohistochemistry (IHC) on Human colon carcinoma (SPC-103) Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Human, Rat brain cell lysates (SPC-103) Rabbit Anti-HSP70 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-103) Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Mouse Pam212 cells (SPC-103) Rabbit Anti-Hsp70 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-103) Rabbit Anti-HSP70 Antibody used in Immunohistochemistry (IHC) on Mouse Inflamed colon (SPC-103)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。