产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
HSP60抗体
目录 :
SPC-105D
规格 :
100微克
价格 :
273.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀
更多信息或购买 :
文章摘录数: 2
参考文献
Brockmann S, Freischmidt A, Oeckl P, Müller K, Ponna S, Helferich A, et al. CHCHD10 mutations p.R15L and p.G66V cause motoneuron disease by haploinsufficiency. Hum Mol Genet. 2018;27:706-715 pubmed 出版商
Peksel B, Gombos I, Peter M, Tiszlavicz Á, Brameshuber M, Balogh G, et al. Mild heat induces a distinct "eustress" response in Chinese Hamster Ovary cells but does not induce heat shock protein synthesis. Sci Rep. 2017;7:15643 pubmed 出版商
图像
图像 1 :
StressMarq Biosciences SPC-105D 图像 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody (SPC-105). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody (SPC-105) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
产品信息
目录号 :
SPC-105D
产品名称 :
HSP60抗体
描述 :
兔抗人类HSP60多克隆
靶标 :
HSP60
共轭标签 :
未共轭
2021标价 :
273.00美元
Currency :
美元
研究领域(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Tags and Cell Markers Organelle Markers
别称(s) :
CPN60抗体, GROEL抗体, HLD4抗体, HSP 60抗体, HSP65抗体, HSPD1抗体, HuCHA60抗体, SPG 13抗体
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
Human HSP60 produced through recombinant DNA methods in E.coli
免疫原物种 :
人类
登录号 :
NP_002147.2
Swiss Prot数据库 :
P10809
应用 :
免疫印迹免疫组化免疫沉淀酶联免疫吸附测定
宿主物种 :
物种活性缩写 :
Hu Ms Rt Bv Dg Ck Rb Hm
物种活性全称 :
人类小鼠大鼠牛狗鸡兔仓鼠
抗体稀释 :
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质A纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~60kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-105D, RRID: AB_10807230
化验分析证明书 :
1 µg/ml of SPC-105 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
Cellular Localization :
Mitochondrion Mitochondrion Matrix
科研背景 :
In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
参考文献 :
1. Hartl, F.U. (1996) Nature 381: 571-579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366. 3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858. 4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. 10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556. 11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-105_Hsp60_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png
SPC-105_HSP60_Antibody_WB_Human_Dog_Mou
se_SKBR3_MDCK_and-MEF-cell-line-lysates
_1.png
SPC-105_Hsp60_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-105_HSP60_Antibody_IHC_Human_Adrenal_1.png
Image Alternative Text :
Rabbit Anti-Hsp60 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-105) Rabbit Anti-HSP60 Antibody used in Immunohistochemistry (IHC) on Adrenal (SPC-105) Rabbit Anti-HSP60 Antibody used in Western blot (WB) on Human, Dog, Mouse SKBR3, MDCK, and MEF cell line lysates (SPC-105) Rabbit Anti-Hsp60 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-105)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。