抗体

HO-1抗体

SPC-112D

100微克

250.00美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠, 犬

免疫印迹, 免疫组化, 免疫细胞化学

HO-1抗体

兔抗人类HO-1多克隆

HO-1

未共轭

250.00美元

美元

Cancer Oxidative Stress Neuroscience Neurodegeneration Alzheimer's Disease Cardiovascular System Blood Platelets Cell Signaling Epigenetics and Nuclear Signaling Nuclear Signaling Pathways NFkB Pathway Cardiovascular System Atherosclerosis Vascular Inflammation Inflammatory Mediators Cardiovascular System Vasculature Endothelium Cancer Cancer Metabolism Response to Hypoxia Cell Signaling Metabolism Metabolism processes Hypoxia

HSP32 Antibody, HMOX1 Antibody, Heme oxygenase 1 Antibody, HO Antibody, HO1 Antibody, , 32 kD antibody, bK286B10 antibody, D8Wsu38e antibody, heat shock protein 32 kD antibody, heat shock protein 32kD antibody, Heat shock protein antibody, Heme oxygenase (decycling) 1 antibody, Heme oxygenase 1 antibody, Hemox antibody, HMOX 1 antibody, Hmox antibody, Hmox1 antibody, HMOX1_HUMAN antibody, HO 1 antibody, HO antibody, HO-1 antibody, HO1 antibody, Hsp32 antibody

100微克

抗体

多克隆

Human heme-oxygenase (HO-1) synthetic multiple antigenic peptide

人类

NP_002124.1

P09601

免疫印迹免疫细胞化学/IF免疫组化

兔

Hu Ms Rt Dg

人类小鼠大鼠狗

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

蛋白质A纯化

PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠

1毫克/毫升

Detects ~32kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-112D, RRID: AB_2248402

1 µg/ml of SPC-112 was sufficient for detection of HO-1 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

内质网微粒体

Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411–419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925–10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759–1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129–135.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-112_HO-1_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite2.png SPC-112_HO-1_Antibody_WB_Human_Cell-line-lysates_1.png SPC-112_HO-1_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png SPC-112_HO-1_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png

Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-112) Rabbit Anti-HO-1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-112) Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-112) Rabbit Anti-HO-1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-112)