抗体

SOD (Mn) Antibody

SPC-118D

100微克

314.00美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠, 犬, 牛

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀

SOD (Mn) Antibody

Rabbit Anti-Human SOD (Mn) Polyclonal

SOD2

未共轭

314.00美元

美元

Cancer Oxidative Stress Cell Signaling Neuroscience Neurodegeneration Cardiovascular System

Manganese SOD Antibody, IPO B Antibody, Mn SOD Antibody, SOD2 Antibody

100微克

抗体

多克隆

Human Mn SOD

人类

NP_000627.2

P04179

免疫印迹免疫组化免疫沉淀酶联免疫吸附测定

兔

Hu Rt Ms Bv Dg Ck Gb GP Pg Hm Mk Rb Sh Xe Fr

Human Rat Mouse Bovine Dog Chicken Gerbil Guinea Pig (Cavia porcellus) Pig Hamster Monkey Rabbit Sheep African clawed frog (Xenopus laevis) Frog Wood Frog (Rana sylvatica)

WB (1:5000), IHC (1:100); optimal dilutions for assays should be determined by the user.

蛋白质A纯化

PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠

1毫克/毫升

Detects ~25kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-118D, RRID: AB_2191654

0.2 µg/ml of SPC-118 was sufficient for detection of Mn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary antibody.

Mitochondrion Mitochondrion Matrix

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102. 2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 3. Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6. 4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925. 5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385. 6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194 7. Wispe J.R., et al. (1989) BBA. 994: 30-36. 8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-118_SOD2_Antibody_IHC_Rat_muscle-fibres_1.png SPC-118_SOD-Mn_Antibody_WB_Mouse-Brain_Rat-Brain-Rat-Brain-Membrane_1.png

Rabbit Anti-SOD2 Antibody used in Immunohistochemistry (IHC) on Rat muscle fibres (SPC-118) Rabbit Anti-SOD (Mn) Antibody used in Western blot (WB) on Brain and Brain Membrane (SPC-118)