抗体

Erk1/2抗体

SPC-120D

100微升

256.00美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠, 牛

免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪

Erk1/2抗体

兔抗大鼠Erk1/2多克隆

ERK1

未共轭

256.00美元

美元

Cell Signaling Post-translational Modifications Phosphorylation Neuroscience Neurodegeneration Alzheimer's Disease

ERK1抗体, ERK2抗体, ERT1抗体, ERT2抗体, 地图kinase1抗体, 地图kinase2抗体, MAPK1抗体, MAPK2抗体, MAPK3抗体, p38抗体, p40抗体, p41抗体, p41mapk抗体, p42 MAPK抗体, p44 ERK1抗体, p44 MAPK抗体, PRKM1 PRKM2抗体, PRKM3抗体

100微升

抗体

多克隆

A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.

大鼠

NP_059043.1

P21708

免疫印迹免疫组化免疫细胞化学/IF FCM

兔

Hu Ms Rt Bv Sh Ck Dr Xe

人类小鼠大鼠牛羊鸡果蝇(黑腹果蝇)非洲爪蛙(非洲爪蛙)

WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.

肽亲和纯化

PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠

1毫克/毫升

Detects ~44kda (ERK1) and ~42kDa (ERK2).

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-120D, RRID: AB_2266080

A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cytoplasm Nucleus

The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44. 3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345. 4. Chuerland D., Marmor G., Shainskaya A. and Seger R. (2008) J Biol Chem. Epub: http://www.jbc.org/cgi/doi/10.1074/jbc.M709030200 5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol Exp Ther 319:991-997.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-120_Erk1-2_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-120_ERK1_Antibody_WB_Human_Cell-line-lysates_1.png SPC-120_ERK1_Antibody_IHC_Mouse_Inflamed-colon_1.png SPC-120_ERK1_Antibody_ICC-IF_Human_HaCaT-cells_4.png SPC-120_ERK1_Antibody_IHC_Mouse_backskin_6.png SPC-120_Erk1-2_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Rabbit Anti-Erk1/2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-120) Rabbit Anti-ERK1 Antibody used in Western blot (WB) on Human Cell line lysates (SPC-120) Rabbit Anti-ERK1 Antibody used in Immunohistochemistry (IHC) on Mouse Inflamed colon (SPC-120) Rabbit Anti-ERK1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-120) Rabbit Anti-ERK1 Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-120) Rabbit Anti-Erk1/2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-120)