产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
鼠双微基因2抗体
目录 :
SPC-1329D
规格 :
100微升
价格 :
255.00美元
克隆性 :
多克隆
宿主 :
共轭标签 :
未共轭
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
StressMarq Biosciences SPC-1329D 图像 1
Western blot analysis of Human SKOV3 cell lysates showing detection of ~90kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329). Lane 1: Human SKOV3 cells. Lane 2: Human SKOV3 cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody (SPC-1329) at 1:1000. Predicted/Observed Size: ~90kDa.
产品信息
目录号 :
SPC-1329D
产品名称 :
鼠双微基因2抗体
描述 :
兔抗人类鼠双微基因2多克隆
靶标 :
鼠双微基因2
共轭标签 :
未共轭
标价 :
255.00美元
Currency :
美元
研究领域(s) :
Cancer Cell Cycle p53 Pathway Cell Signaling Epigenetics and Nuclear Signaling Transcription Cell Signaling Post-translational Modifications Ubiquitination E3 Ubiquitin Ligases
别称(s) :
ACTFS antibody, Double minute 2 protein antibody, E3 ubiquitin-protein ligase Mdm2 antibody, Hdm 2 antibody, Hdm2 antibody, HDMX antibody, MDM 2 antibody, MDM2 antibody, MDM2 oncogene E3 ubiquitin protein ligase antibody, Mdm2 p53 E3 ubiquitin protein ligase homolog antibody, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody, MDM2_HUMAN antibody, MDM2BP antibody, Mouse Double Minute 2 antibody, MTBP antibody, Murine Double Minute Chromosome 2 antibody, Oncoprotein Mdm2 antibody, p53 Binding Protein Mdm2 antibody, p53-binding protein Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody, Ubiquitin protein ligase E3 Mdm2 antibody
规格 :
100微升
类别 :
抗体
产品类型 :
多克隆
免疫原 :
Synthesized peptide derived from human MDM2.
免疫原物种 :
人类
登录号 :
NP_002383.2
Swiss Prot数据库 :
Q00987
应用 :
免疫印迹免疫组化免疫细胞化学/IF酶联免疫吸附测定SPR
宿主物种 :
抗体亚型 :
IgG
物种活性 :
人类大鼠
抗体稀释 :
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:40000); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS pH值7.4, 50% 甘油, 150mM氯化钠, 0.02% 叠氮化钠
特异性 :
检测内生水平总数鼠双微基因2蛋白
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-1329D, RRID: AB_2713709
化验分析证明书 :
A 1:1000 dilution of SPC-1329 was sufficient for detection of MDM2 in 10 µg of SKOV3 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Nucleus Cytoplasm
科研背景 :
Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene. MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
参考文献 :
1. Uhrinova S et al. (2005) J. Mol. Biol. 350 (3): 587–98. 2. Oliner JD et al. (1992) Nature 358 (6381): 80–3. 3. Wade M. et al. (2006) J. Biol. Chem. 281 (44): 33036–44. 4. Ebrahim, M. et al. (2015) Histology and histopathology, 30(11), 1271-1282. 30(11):1271-1282.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-1329_MDM2_Antibody_IHC_Human_Breast-Carcinoma-Tissue_1.png SPC-1329_MDM2_Antibody_ICC-IF_Human_A549_1.png SPC-1329_MDM2_Antibody_WB_Human_SKOV3-cells_1.png
Image Alternative Text :
Rabbit Anti-MDM2 Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1329) Rabbit Anti-MDM2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Lung carcinoma epithelial cell line (A549) (SPC-1329) Rabbit Anti-MDM2 Antibody used in Western blot (WB) on SKOV3 cell lysates (SPC-1329)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。