抗体

Akt2(PKB乙)抗体

SPC-148F

400微升

263.00美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠, 犬, 牛

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学

Akt2(PKB乙)抗体

兔抗人类Akt2(PKB乙)多克隆

AKT2

未共轭

263.00美元

美元

Cell Signaling Post-translational Modifications Phosphorylation

AKT2抗体, PKB抗体, PKB乙抗体, PRKBB抗体, 蛋白激酶Akt2抗体, RAC乙抗体

400微升

抗体

多克隆

A five residue synthetic peptide from the C-terminal of human AKT2, coupled to KLH

人类

NP_001617.1

P31751

免疫印迹免疫细胞化学/IF酶联免疫吸附测定

兔

Hu Ms Rt Bv Ck Dg GP Hm Mk Pg Rb Sh Xe

Human Mouse Rat Bovine Chicken Dog Guinea Pig (Cavia porcellus) Hamster Monkey Pig Rabbit Sheep African clawed frog (Xenopus laevis)

WB (1:1000), ICC/IF (1:25); optimal dilutions for assays should be determined by the user.

肽亲和纯化

TBS, 50% 甘油, 0.01% 叠氮化钠

0.25毫克/毫升

Detects ~65kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3).

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-148F, RRID: AB_2225163

A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Cytoplasm Nucleus Cell Membrane

Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).

1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7. 2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64. 3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95. 4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-148_AKT2_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-148_AKT2_Antibody_WB_multiple_Cell-line-lysates_1.png

Rabbit Anti-AKT2 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-148) Rabbit Anti-AKT2 Antibody used in Western blot (WB) on multiple Cell line lysates (SPC-148)