抗体

磷酸酪氨酸抗体

SPC-161F

400微升

310.00美元

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀

磷酸酪氨酸抗体

兔抗磷酸酪氨酸多克隆

磷酸酪氨酸

未共轭

310.00美元

美元

Cell Signaling Post-translational Modifications Phosphorylation

磷酸化酪氨酸抗体

400微升

抗体

多克隆

磷酸酪氨酸,KLH偶联

免疫印迹免疫组化免疫细胞化学/IF免疫沉淀酶联免疫吸附测定

兔

都是

Species Independent

WB (1:500), IHC (1:100), ICC/IF (1:50), ELISA (1:2000), IP (1:100); optimal dilutions for assays should be determined by the user.

肽亲和纯化

PBS pH7.0, 50% 甘油, 0.01% 叠氮化钠

0.25毫克/毫升

Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-161F, RRID: AB_854256

A 1:250 dilution of SPC-161 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).

1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365. 3. Downward J. (2001) Nature 411: 759-762. 4. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678. 5. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol Cell Biol. 3: 1343-1352. 6. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature 294: 654-656. 7. Ostrovsky PC. (1995) Genes Dev. 9(16): 2034-2041.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-163_Phosphotyrosine_Antibody_IHC_Hu
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SPC-161_Phosphotyrosine_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-161_Phosphotyrosine_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png

Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-161) Rabbit Anti-Phosphotyrosine Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-161) Rabbit Anti-Phosphotyrosine Antibody used in Immunohistochemistry (IHC) on Human breast cancer (SPC-161)