抗体

GSK-3beta抗体

SPC-169D

100微克

252.00美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠, 牛

免疫印迹, 免疫组化, 免疫细胞化学

GSK-3beta抗体

兔抗人类GSK-3beta多克隆

GSK3乙

未共轭

252.00美元

美元

Cell Signaling Post-translational Modifications Phosphorylation

Glycogen Synthase kinase 3 beta Antibody, GSK 3 beta Antibody, GSK 3B Antibody, GSK-3 beta Antibody, GSK3B Antibody, GSK3B_Human Antibody, GSK3beta isoform Antibody

100微克

抗体

多克隆

Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3Beta

人类

NP_001139628.1

P49841

免疫印迹免疫组化免疫细胞化学/IF

兔

Hu Ms Rt Bv

人类小鼠大鼠牛

WB (1:2000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

蛋白质A纯化

PBS pH7.4

1.3毫克/毫升

Detects ~47kDa.

-20ºC

Blue Ice or 4ºC

StressMarq Biosciences Cat# SPC-169D, RRID: AB_2703785

1 µg/ml of SPC-169 was sufficient for detection of GSK-beta in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Cytoplasm Nucleus Cell Membrane

Glycogen synthase kinase 3, is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues.  It has also been shown to regulate immune and migratory processes, and is tied to pathways of cell proliferation and apoptosis (1-3). In mammals, it is encoded by two genes- GSK-3Alpha and GSK-3Beta. They are structurally similar, but functionally non-identical. GSK-3Alpha is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3Beta negatively regulates cardiac hypertrophy and cardiac development through its effect on WNT signaling (4). GSK-3 alpha and GSK-3 beta share 85% amino acid identity. GSK-3 has been implicated in a number of diseases including Type II diabetes, Alzheimer's, cancer and bipolar (4, 5).

1. Jope R.S. (2007) Neurochem Res. 32(4-5): 577-595. 2. Wang H. (2011) Cytokind. 53(2): 130-140. 3. Mills C.N. (2011) Front Mol Neurosci. 47(4): 47. 4. Hardt S.E., Sadoshima J. (2002) Circ Res. 90(10): 1055-1063. 5. Doble D.W., Woodgett J.R. (2003) J Cell Sci. 116(Pt7): 1175-1186.

Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

SPC-169_GSK3beta_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_100x_Composite.png SPC-169_GSK3-Beta_Antibody_WB_Human_Cell-line-lysates_1.png SPC-169_GSK3beta_Antibody_ICC-IF_Human_Heat-Shocked-HeLa-Cells_20x_Composite.png

Rabbit Anti-GSK3beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-169) Rabbit Anti-GSK3 Beta Antibody used in Western blot (WB) on Human Cell line lysates (SPC-169) Rabbit Anti-GSK3beta Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked Cervical cancer cell line (HeLa) (SPC-169)