产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
p38抗体
目录 :
SPC-172D
规格 :
100微升
价格 :
209.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, ,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 1
参考文献
Ta H, Le T, Ishii H, Takarada Iemata M, Hattori T, Hashida K, et al. Atf6? deficiency suppresses microglial activation and ameliorates pathology of experimental autoimmune encephalomyelitis. J Neurochem. 2016;139:1124-1137 pubmed 出版商
图像
图像 1 :
StressMarq Biosciences SPC-172D 图像 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-p38 Polyclonal Antibody (SPC-172). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-p38 Polyclonal Antibody (SPC-172) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion. Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-p38 Antibody. (C) Composite.
产品信息
目录号 :
SPC-172D
产品名称 :
p38抗体
描述 :
兔抗人类p38多克隆
靶标 :
p38
共轭标签 :
未共轭
2021标价 :
209.00美元
Currency :
美元
研究领域(s) :
Cell Signaling Post-translational Modifications Phosphorylation Cancer
别称(s) :
csaid结合蛋白1抗体, CSBP1抗体, CSBP2抗体, EXIP抗体, 地图激酶MXI2抗体, MAPkinase p38alpha抗体, MAPK14抗体, p38甲抗体, p38 MAP激酶抗体, p丝裂原活化蛋白激酶抗体, RK抗体, SAPK 2A抗体, 应激活化蛋白激酶2a抗体
规格 :
100微升
类别 :
抗体
产品类型 :
多克隆
免疫原 :
A 20 residue synthetic peptide based on the human p38 with the cysteine residue added and coupled to KLH
免疫原物种 :
人类
登录号 :
NP_001306.1
Swiss Prot数据库 :
Q16539
应用 :
免疫印迹免疫组化免疫细胞化学/IF免疫沉淀
宿主物种 :
物种活性缩写 :
Hu Ms Rt Bv Mk Rb Pg Dg Hm Ck Sh GP
物种活性全称 :
Human Mouse Rat Bovine Monkey Rabbit Pig Dog Hamster Chicken Sheep Guinea Pig (Cavia porcellus)
抗体稀释 :
WB (1:1000), ICC/IF (1:100), IP (1:250); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~43kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-172D, RRID: AB_1055444
化验分析证明书 :
A 1:1000 dilution of SPC-172 was sufficient for detection of p38 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
Cellular Localization :
Cytoplasm Nucleus
科研背景 :
The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, protein-serine/threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs); extracellular signal-regulated kinases (ERKs); and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
参考文献 :
1. Pearson G., et al (2001) Endocrine Reviews 22 (2): 153-183. 2. Fan Y., et al (2007) Mol. Cells 23 (1): 30-38. 3. Han J., et al. (1994) Science 265: 808-811. 4. Van L. A., et al. (2004) Faseb J. 18: 1946−1948. 5. Deng et al. (2003) Cell 115: 61-70. 6. Salojin K.V., et al. (2006) J Immunol. 176 (3):1899-907. 7. Medicherla S., et al. (2006) J Pharmacol Exp Ther. 318(1): 99-107.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-172_p38_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-172_p38_Antibody_WB_Human_cancer-cell-lines_1.png SPC-172_p38_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png
Image Alternative Text :
Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-172) Rabbit Anti-p38 Antibody used in Western blot (WB) on Human cancer cell lines (SPC-172) Rabbit Anti-p38 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-172)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。