产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
RAS抗体
目录 :
SPC-173D
规格 :
100微克
价格 :
257.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 牛
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
StressMarq Biosciences 产品网页
产品信息
目录号 :
SPC-173D
产品名称 :
RAS抗体
描述 :
兔抗人类RAS多克隆
靶标 :
RAS
共轭标签 :
未共轭
2021标价 :
257.00美元
Currency :
美元
研究领域(s) :
Cancer Cell Signaling Epigenetics and Nuclear Signaling
别称(s) :
GTPase Hras Antibody, GTPase Kras Antibody, GTPase Nras Antibody, Ha Ras Antibody, K Ras Antibody, K ras p21 Antibody, KRAS Antibody, NRAS Antibody, p21ras Antibody, RASH2 Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
免疫原物种 :
人类
登录号 :
NP_035067.2
Swiss Prot数据库 :
B2KGV5
应用 :
免疫印迹免疫组化免疫细胞化学/IF
宿主物种 :
兔
物种活性缩写 :
Hu Ms Rt Bv
物种活性全称 :
人类小鼠大鼠牛
抗体稀释 :
WB (1:1000), IHC (1:100), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质G纯化
储存缓冲液 :
PBS pH7.4, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~21kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-173D, RRID: AB_2703838
化验分析证明书 :
1 µg/ml of SPC-173 was sufficient for detection of Ras in 20 µg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
细胞膜高尔基体高尔基体膜
科研背景 :
The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
参考文献 :
1. Boguski M.S. and McCormick F. (1993) Nature 366: 643–654. 2. Avruch J., et al. (1994) Trends Biochem. Sci.19: 279–283. 3. Buday L. and Downward J. (1993) Cell 73: 611–620. 4. Huang D.C., et al. (1993) Mol. Cell Biol. 13: 2420–2431. 5. Bos J.L. (1989) Cancer Res. 49: 4682–4689. 6. Ma J. and Karplus M. (1997) J. Mol. Biol. 274:114–131.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-173_RAS_Antibody_ICC-IF_Human_HeLa-Cells_100x_Composite.png SPC-173_RAS_Antibody_IHC_Mouse_backskin_1.png SPC-173_RAS_Antibody_WB_Mouse_Brain-Cortex-cell-lysates_1.png SPC-173_RAS_Antibody_ICC-IF_Human_HaCaT-cells_1.png SPC-173_RAS_Antibody_ICC-IF_Human_HeLa-Cells_20x_Composite.png
Image Alternative Text :
Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-173) Rabbit Anti-RAS Antibody used in Immunohistochemistry (IHC) on Mouse backskin (SPC-173) Rabbit Anti-RAS Antibody used in Western blot (WB) on Brain Cortex cell lysates (SPC-173) Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SPC-173) Rabbit Anti-RAS Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Cervical cancer cell line (HeLa) (SPC-173)
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。