产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
HSP70/HSC70(植物)抗体
目录 :
SPC-312D
规格 :
100微升
价格 :
314.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
StressMarq Biosciences SPC-312D 图像 1
Western blot analysis of Rat (1) and Human (2) Tissue lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-312). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-312) at 1:1000.
图像 2 :
StressMarq Biosciences SPC-312D 图像 2
Western blot analysis of Rat (1) and Human (2) Tissue lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (SPC-312). Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (SPC-312) at 1:1000.
产品信息
目录号 :
SPC-312D
产品名称 :
HSP70/HSC70(植物)抗体
描述 :
兔抗HSP70/HSC70(植物)多克隆
靶标 :
HSP70
共轭标签 :
未共轭
2021标价 :
314.00美元
Currency :
美元
研究领域(s) :
Cancer Heat Shock Cell Signaling Protein Trafficking Chaperone Proteins Cancer Tumor Biomarkers
别称(s) :
HSC54抗体, HSC71抗体, HSC73抗体, HSP71抗体, HSP73抗体, HSPA10抗体, HSPA8抗体, LAP1抗体, NIP71抗体, HSC70抗体
规格 :
100微升
类别 :
抗体
产品类型 :
多克隆
免疫原 :
KLH-conjugated synthetic peptide conserved in all known sequences of HSP70 and HSC70 proteins
应用 :
免疫印迹
宿主物种 :
物种活性缩写 :
Pl Ar Fs Mm
物种活性全称 :
Plant Arabidopsis thaliana Radish (Raphanus sativus) Barley (Hordeum vulgare L.) Lettuce (Lactuca sativa) Fish Mammals
抗体稀释 :
WB (1:1000), IP (1:1000); optimal dilutions for assays should be determined by the user.
纯化 :
兔抗血清
储存缓冲液 :
Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.
特异性 :
Detects ~70kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-312D, RRID: AB_1501444
化验分析证明书 :
0.1 µl/ml of SPC-312 was sufficient for detection of HSP70/HSC70 in 1 mg wet plant sample extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
细胞质
科研背景 :
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.
参考文献 :
1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 2. Rothman J. (1989) Cell. 59: 591-601. 3. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-312_HSP70_Antibody_WB_Rat-1-and-Human-2_Tissue-lysates_1.png
Image Alternative Text :
Rabbit Anti-HSP70 Antibody used in Western blot (WB) on Rat (1) and Human (2) Tissue lysates (SPC-312)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。