产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
ENaC丙抗体
目录 :
SPC-405D
规格 :
100微克
价格 :
310.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
StressMarq Biosciences 产品网页
文章摘录数: 13
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:1000; 图 5c
Ferdaus M, Barber K, López Cayuqueo K, Terker A, Argaiz E, Gassaway B, et al. SPAK and OSR1 play essential roles in potassium homeostasis through actions on the distal convoluted tubule. J Physiol. 2016;594:4945-66 pubmed 出版商
Khedr S, Palygin O, Pavlov T, Blass G, Levchenko V, Alsheikh A, et al. Increased ENaC activity during kidney preservation in Wisconsin solution. BMC Nephrol. 2019;20:145 pubmed 出版商
Liu M, Zhu Y, Sun Y, Wen Z, Huang S, Ding G, et al. MnTBAP therapy attenuates the downregulation of sodium transporters in obstructive kidney disease. Oncotarget. 2018;9:394-403 pubmed 出版商
Zhang C, Wang L, Su X, Zhang J, Lin D, Wang W. ENaC and ROMK activity are inhibited in the DCT2/CNT of TgWnk4PHAII mice. Am J Physiol Renal Physiol. 2017;312:F682-F688 pubmed 出版商
Xu N, Hirohama D, Ishizawa K, Chang W, Shimosawa T, Fujita T, et al. Hypokalemia and Pendrin Induction by Aldosterone. Hypertension. 2017;69:855-862 pubmed 出版商
You H, Ge Y, Zhang J, Cao Y, Xing J, Su D, et al. Derlin-1 promotes ubiquitylation and degradation of the epithelial Na+ channel, ENaC. J Cell Sci. 2017;130:1027-1036 pubmed 出版商
Klemens C, Edinger R, Kightlinger L, Liu X, Butterworth M. Ankyrin G Expression Regulates Apical Delivery of the Epithelial Sodium Channel (ENaC). J Biol Chem. 2017;292:375-385 pubmed 出版商
Xu C, Lu A, Wang H, Fang H, Zhou L, Sun P, et al. (Pro)Renin receptor regulates potassium homeostasis through a local mechanism. Am J Physiol Renal Physiol. 2017;313:F641-F656 pubmed 出版商
Pavlov T, Levchenko V, Ilatovskaya D, Moreno C, Staruschenko A. Renal sodium transport in renin-deficient Dahl salt-sensitive rats. J Renin Angiotensin Aldosterone Syst. 2016;17: pubmed 出版商
Reed Geaghan E, Wright M, See L, Adelman P, Lee K, Koerber H, et al. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes. J Neurosci. 2016;36:4362-76 pubmed 出版商
Ramkumar N, Stuart D, Mironova E, Bugay V, Wang S, Abraham N, et al. Renal tubular epithelial cell prorenin receptor regulates blood pressure and sodium transport. Am J Physiol Renal Physiol. 2016;311:F186-94 pubmed 出版商
Patel Chamberlin M, Varasteh Kia M, Xu J, Barone S, Zahedi K, Soleimani M. The Role of Epithelial Sodium Channel ENaC and the Apical Cl-/HCO3- Exchanger Pendrin in Compensatory Salt Reabsorption in the Setting of Na-Cl Cotransporter (NCC) Inactivation. PLoS ONE. 2016;11:e0150918 pubmed 出版商
Xu W, Huang Y, Li L, Sun Z, Shen Y, Xing J, et al. Hyperuricemia induces hypertension through activation of renal epithelial sodium channel (ENaC). Metabolism. 2016;65:73-83 pubmed 出版商
图像
图像 1 :
StressMarq Biosciences SPC-405D 图像 1
Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody (SPC-405). Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody (SPC-405) at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.
产品信息
目录号 :
SPC-405D
产品名称 :
ENaC丙抗体
描述 :
兔抗大鼠ENaC丙多克隆
靶标 :
ENaC
共轭标签 :
未共轭
2021标价 :
310.00美元
Currency :
美元
研究领域(s) :
Neuroscience Neurotransmission Ion Channels Sodium Channels Epithelial Sodium Channels (ENaC)
别称(s) :
SCNN1G Antibody, Epithelial Sodium Channel gamma Antibody, Amiloride sensitive epithelial sodium channel gamma subunit Antibody, Amiloride sensitive sodium channel subunit gamma Antibody, Amiloride-sensitive sodium channel subunit gamma Antibody, BESC3 Antibody, ENaC gamma subunit Antibody, ENaCG Antibody, ENaCgamma Antibody, Epithelial Na(+) channel subunit gamma Antibody, Epithelial Na+ channel subunit gamma Antibody, Gamma ENaC Antibody, Gamma NaCH Antibody, Gamma-ENaC Antibody, Gamma-NaCH Antibody, Nonvoltage gated sodium channel 1 subunit gamma Antibody, Nonvoltage-gated sodium channel 1 subunit gamma Antibody, PHA 1 Antibody, PHA1 Antibody, SCNEG Antibody, SCNN 1G Antibody, SCNN1G Antibody, SCNNG_HUMAN Antibody, Sodium channel non voltage gated 1 gamma subunit Antibody, Sodium channel nonvoltage gated 1 gamma Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
免疫原物种 :
大鼠
登录号 :
NP_058742
Swiss Prot数据库 :
P37091
应用 :
免疫印迹免疫组化免疫细胞化学/IF
宿主物种 :
兔
物种活性缩写 :
Rt Ms Xe Hm Hu
物种活性全称 :
大鼠小鼠非洲爪蛙(非洲爪蛙)仓鼠人类
抗体稀释 :
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
蛋白质A纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~83kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-405D, RRID: AB_10640369
化验分析证明书 :
1 µg/ml of SPC-405 was sufficient for detection of gamma-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Apical Cell Membrane
科研背景 :
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, α, β, and γ. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
参考文献 :
1. Kakizoe Y., et al. (2009) J Hypertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-405_ENaC_Antibody_IHC_Rat_kidney-tissue_1.png SPC-405_ENaC_Antibody_WB_Rat_kidney-tissue-lysates_1.png SPC-405_ENaC_Antibody_WB_Mouse_kidney-cortex_1.png
Image Alternative Text :
Rabbit Anti-ENaC Antibody used in Immunohistochemistry (IHC) on Rat kidney tissue (SPC-405) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Rat kidney tissue lysates (SPC-405) Rabbit Anti-ENaC Antibody used in Western blot (WB) on Mouse kidney cortex (SPC-405)
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。