产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
LC3A抗体
目录 :
SPC-613D
规格 :
100微克
价格 :
301.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
StressMarq Biosciences 产品网页
图像
图像 1 :
StressMarq Biosciences SPC-613D 图像 1
Western blot analysis of Rat Liver cell lysates showing detection of 14 kDa LC3A protein using Rabbit Anti-LC3A Polyclonal Antibody (SPC-613). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-LC3A Polyclonal Antibody (SPC-613) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.
产品信息
目录号 :
SPC-613D
产品名称 :
LC3A抗体
描述 :
兔抗人类LC3A多克隆
靶标 :
LC3A
共轭标签 :
未共轭
2021标价 :
301.00美元
Currency :
美元
研究领域(s) :
Cancer Autophagy Cell Signaling Neuroscience Cardiovascular System Heart
别称(s) :
MAP1 light chain 3 like protein 1 Antibody, Map1lc3a Antibody, MAP1BLC3 Antibody, Autophagy-related ubiquitin-like modifier LC3 A Antibody, MAP1LC3A Antibody, LC3A Antibody, Microtubule-associated proteins 1A and 1B, light chain 3 Antibody, MAP1ALC3 Antibody, MAP1A/1B light chain 3 A Antibody, MAP1A/MAP1B light chain 3 A Antibody, Microtubule-associated protein 1 light chain 3 alpha Antibody, LC3 Antibody, Microtubule-associated proteins 1A/1B light chain 3A Antibody, Autophagy-related protein LC3 A Antibody, MAP1A/MAP1B LC3 A Antibody, ATG8E Antibody, MLP3A_HUMAN Antibody, MAP1 light chain 3-like protein 1 Antibody, Microtubule associated proteins 1A/1B light chain 3 Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
合成肽来自N末端人的LC3A(氨基酸。 1-12)
免疫原物种 :
人类
登录号 :
NP_115903.1
Swiss Prot数据库 :
Q9H492
应用 :
免疫印迹免疫细胞化学/IF
宿主物种 :
兔
物种活性缩写 :
Hu Ms Rt
物种活性全称 :
人类小鼠大鼠
抗体稀释 :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~14 kDa. Band at 50 kDa is LC3A complex.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-613D, RRID: AB_2704943
化验分析证明书 :
A 1:1000 dilution of SPC-613 was sufficient for detection of LC3A in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cytoskeleton Endomembrane System Lipid-Anchor Cytoplasmic Vesicle Autophagosome Membrane Lipid-Anchor Cytoplasmic Vesicle Autophagosome
组织特异性 :
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
科研背景 :
Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy; once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
参考文献 :
1. Tanida I., Ueno T., & Kominami E. (2008) Methods Mol Biol. 445:77-88. 2. He H., et al. (2003) J Biol Chem. 278(31): 29278-87.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-613_LC3A_Antibody_ICC-IF_Human_SK-N-BE-Cells-Human-Neuroblastoma-cells_60X_Composite_1.png SPC-613-LC3A-Antibody-WB-Rat-Liver-cell-lysates-1.png
Image Alternative Text :
Rabbit Anti-LC3A Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma cell line (SK-N-BE) (SPC-613) Rabbit Anti-LC3A Antibody used in Western blot (WB) on Liver cell lysates (SPC-613)
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。