产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
GABARAPL1抗体
目录 :
SPC-623D
规格 :
100微克
价格 :
301.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
StressMarq Biosciences 产品网页
图像
图像 1 :
StressMarq Biosciences SPC-623D 图像 1
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~14kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-623). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-623) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~14kDa.
产品信息
目录号 :
SPC-623D
产品名称 :
GABARAPL1抗体
描述 :
兔抗人类GABARAPL1多克隆
靶标 :
GABARAPL1
共轭标签 :
未共轭
2021标价 :
301.00美元
Currency :
美元
研究领域(s) :
Neuroscience Neurotransmission Neurotransmitter Receptors GABA Receptors Cancer Autophagy
别称(s) :
APG8 like Antibdoy, ATG8 Antibody, GABA(A) Receptor Associated Protein Like 1 Antibody, Glandular epithelial cell protein 1 Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
合成肽来自C末端人的GABARAPL1
免疫原物种 :
人类
登录号 :
NP_113600.1
Swiss Prot数据库 :
Q9H0R8
应用 :
免疫印迹免疫细胞化学/IF
宿主物种 :
兔
物种活性缩写 :
Hu Rt
物种活性全称 :
人类大鼠
抗体稀释 :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Detects ~14-16kDa. Other observed bands at ~32kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-623D, RRID: AB_2705015
化验分析证明书 :
A 1:1000 dilution of SPC-623 was sufficient for detection of GABARAPL1 on rat liver lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cytoskeleton Endoplasmic Reticulum Golgi Apparatus Cytoplasmic Vesicle Autophagosome
科研背景 :
GABARAPL1, one of the proteins belonging to the GABARAP (GABA(A) receptor-associated protein) family, is highly expressed in the central nervous system and implicated in processes such as receptor and vesicle transport as well as autophagy. The proteins that make up the GABARAP family demonstrate conservation of their amino acid sequences and protein structures. In humans, GABARAPL1 shares 86% identity with GABARAP and 61% with GABARAPL2 (GATE-16) (1). GABARAPL1, or GABA(A) Receptor-Associated Protein Like 1, is a ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (2).
参考文献 :
1. Le Grand J.N., at al. (2011) Autophagy. 7(11): 1302-1307. 2. Tanida S., et al. (2006) FEBS J. 273(11): 2553-2562.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-623-GABARAPL1-Antibody-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png SPC-623_GABARAPL1_Antibody_WB_Human_HeLa_1.png
Image Alternative Text :
Rabbit Anti-GABARAPL1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-623) Rabbit Anti-GABARAPL1 Antibody used in Western blot (WB) on Cervical cancer cell line (HeLa) lysate (SPC-623)
更多信息或购买 :
StressMarq Biosciences 产品网页
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚，专注于热休克、细胞压力、离子通道及细胞信号研究领域，提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目，特别针对于新的研究领域，鼓励客户在购买之前先测试小规格试用产品。此外，公司提供采用新专利免疫组化技术的试剂盒，采用前所未有的抗体浓度进行实验。