产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
p53抗体
目录 :
SPC-682D
规格 :
100微克
价格 :
301.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
StressMarq Biosciences SPC-682D 图像 1
Western blot analysis of Human A431 showing detection of ~43.7kDa p53 protein using Rabbit Anti-p53 Polyclonal Antibody (SPC-682). Lane 1: MW Ladder. Lane 2: Human A431 (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-p53 Polyclonal Antibody (SPC-682) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~43.7kDa.
产品信息
目录号 :
SPC-682D
产品名称 :
p53抗体
描述 :
Rabbit Anti-Human p53 Polyclona
靶标 :
p53
共轭标签 :
未共轭
2021标价 :
301.00美元
Currency :
美元
研究领域(s) :
Cancer Cell Cycle p53 Pathway Cancer Tumor suppressors Apoptosis Cell Signaling Epigenetics and Nuclear Signaling DNA/RNA DNA Damage and Repair
别称(s) :
TP53 Antibody, Tumor Protein 53 Antibody, BCC7 Antibody, LFS1 Antibody, TRP53 Antibody, p53 tumor suppressor Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
合成肽来自C末端人的肿瘤蛋白p53
免疫原物种 :
人类
登录号 :
NP_000537.3
Swiss Prot数据库 :
P04637
应用 :
免疫印迹免疫细胞化学/IF
宿主物种 :
物种活性缩写 :
Hu
物种活性全称 :
人类
抗体稀释 :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Predicted molecular weight at ~43.7kDa. Observed bands at ~53kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-682D, RRID: AB_2705645
化验分析证明书 :
A 1:1000 dilution of SPC-682 was sufficient for detection of p53 on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Nucleus PML body Endoplasmic Reticulum Mitochondrion matrix
科研背景 :
The p53 protein (tumor protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumor suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5).
参考文献 :
1. Georescu C.V., Saftoiu A., Georgescu C.C., Ciurea R. and Ciurea T. (2007) J Gastrointestin Liver Dis. 16(2): 133-9. 2. Strachan T., et al. (1999) Human Molecular Genetics 2. Ch. 18: Cancer Genetics. 3. Hansen J.E., et al. (2007) Cancer Research 67: 1769-1774. 4. Di Cesare E., et al. (2001) Ann Clin Lab Sci 31: 253-8. 5. Akere A., Otegbayo J.A. (2007) Singapore Med J. 48(1): 41-4.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-682-p53-Antibody-ICC-IF-Human-Cervical-Cancer-cell-line-HeLa-40X-Composite-1.png SPC-682_p53_Antibody_WB_Human_HeLa_293T_1.png SPC-682_p53_Antibody_WB_Human_A431_1.png
Image Alternative Text :
Rabbit Anti-p53 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cervical cancer cell line (HeLa) (SPC-682) Rabbit Anti-p53 Antibody used in Western blot (WB) on HeLa and HEK293T cell lysates (SPC-682) Rabbit Anti-p53 Antibody used in Western blot (WB) on A431 (SPC-682)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。