产品简要
公司名称 :
StressMarq Biosciences
产品类型 :
抗体
产品名称 :
胆碱乙酰转移酶类抗体
目录 :
SPC-706D
规格 :
100微克
价格 :
301.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
StressMarq Biosciences SPC-706D 图像 1
Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (SPC-706) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
产品信息
目录号 :
SPC-706D
产品名称 :
胆碱乙酰转移酶类抗体
描述 :
兔抗人类胆碱乙酰转移酶类多克隆
靶标 :
胆碱乙酰转移酶类
共轭标签 :
未共轭
2021标价 :
301.00美元
Currency :
美元
研究领域(s) :
Neuroscience Neurotransmission Neurotransmitter Receptors Cell Signaling Neurodegeneration Alzheimer's Disease
别称(s) :
CHAT_Human Antibody, Acetyl CoA choline O Acetyltransferase Antibody, Choline Acetylase Antibody, CLAT_Human Antibody, CMS1A Antibody
规格 :
100微克
类别 :
抗体
产品类型 :
多克隆
免疫原 :
Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
免疫原物种 :
人类
登录号 :
NP_001136401.1
Swiss Prot数据库 :
P28329
应用 :
免疫印迹免疫细胞化学/IF
宿主物种 :
物种活性缩写 :
Hu Ms
物种活性全称 :
人类小鼠
抗体稀释 :
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
纯化 :
肽亲和纯化
储存缓冲液 :
PBS, 50% 甘油, 0.09% 叠氮化钠
浓度 :
1毫克/毫升
特异性 :
Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
储存温度 :
-20ºC
运输温度 :
Blue Ice or 4ºC
Cite this Product :
StressMarq Biosciences Cat# SPC-706D, RRID: AB_2705969
化验分析证明书 :
A 1:1000 dilution of SPC-706 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization :
Cytoplasm Cytosol Mitochondrion Nucleus
科研背景 :
Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).
参考文献 :
1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.
Field of Use :
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Image Filenames :
SPC-706_Choline-Acetyltransferase_Antib
ody_WB_Mouse_Brain_1.png
SPC-706-Choline-Acetyltransferase-Antibody-ICC-IF-Human-Neuroblastoma-cell-line-SK-N-BE-60X-Composite-1.png
Image Alternative Text :
Rabbit Anti-Choline Acetyltransferase Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Neuroblastoma cell line (SK-N-BE) (SPC-706) Rabbit Anti-Choline Acetyltransferase Antibody used in Western blot (WB) on Brain (SPC-706)
更多信息或购买 :
公司信息
StressMarq Biosciences
PO Box 55036 CADBORO BAY
3825 Cadboro Bay Road
Victoria BC V8N 4G0
info@stressmarq.com
http://www.stressmarq.com
1-250-294-9065
公司总部: 加拿大
StressMarq Biosciences Inc.是由Dr.Louwrier创建与温哥华的维多利亚,专注于热休克、细胞压力、离子通道及细胞信号研究领域,提供大量经过严格验证的抗体、蛋白及试剂盒。公司致力于提供高质量试剂以满足并超越客户期望。它也推出抗体试用项目,特别针对于新的研究领域,鼓励客户在购买之前先测试小规格试用产品。此外,公司提供采用新专利免疫组化技术的试剂盒,采用前所未有的抗体浓度进行实验。