ELISA/assay

Human PAI-1 Immunoassay Kit

31070

0.031-2.00 ng/ml

429美元

Human PAI-1 Immunoassay Kit

小鼠

单克隆

Detection: Biotin-labeled

酶联免疫吸附试剂盒

0.031-2.00 ng/ml

429美元

This Human PAI-1 ELISA kit is designed for quantification of human PAI-1 in serum and plasma samples.

No cross-reactivity with mouse and rat PAI-1, and other cytokine or hormone molecules including human resistin, TNF , ANGPTL4, insulin, leptin and IL6.

人类特异性

Plasminogen activator inhibitor-1 (PAI-1) is the primary inhibitor of tissue-type and urokinase-type plasminogen activator, playing a major role in fibrinolysis [1-2]. PAI-1 is mainly produced by the endothelium, but is also secreted by other tissue types, such as adipose tissue [3]. It is normally present at low levels in plasma and tissue, but its expression and release increased in various disease states (such as a number of forms of cancer), as well as in obesity and the metabolic syndrome [4]. PAI-1 is also involved in the pathophysiology of renal, pulmonary, cardiovascular, and metabolic diseases [5-8]. Elevated local or systemic PAI-1 can also exacerbate such pathologic conditions.

Serpin family E member 1, PAI, PAI1, PAI-1, PLANH1

人类

5x Assay Buffer & 10x Wash Buffer are provided.

ELISA: This Human PAI-1 ELISA kit is designed for quantification of human PAI-1 in serum and plasma samples.

This assay is a quantitative sandwich ELISA. The immunoplate is precoated with a mouse monoclonal antibody specific for human PAI-1. Standards and samples are pipetted into the wells and any human PAI-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human PAI-1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human PAI-1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human PAI-1, the unknown sample concentration can be interpolated from a reference curve included in each assay.

The kit should be stored at 2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use. Remove any unused antibody-coated strips from the Human PAI-1 microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.

[1] Lijnen HR, et al. (1995) Baillieres Clin Haematol. 8: 277–290 [2] Dellas C, et al. (2005) Thromb Haemost. 2005; 93: 631–640 [3] Binder BR, et al. (2002) News Physiol Sci 17:56-61 [4] Vague P, et al. (1986) Metabolism. 35: 250–253 [5] Sobel Be, et al. (2003) Arterioscler Thromb Vasc Biol. 23: 1979–1989 [6] Eitzman DT, et al. (1996) J Clin Invest. 97(1):232-7 [7] Gils A, et al. (2004) Thromb. Haemost. 91 (3): 425–37 [8] Cale JM, Lawrence DA. (2007) Curr Drug Targets. 8(9):971-81