抗体

CDC2(CDK1, 细胞周期蛋白依赖性激酶 1)

030070

100微升

473美元

单克隆

小鼠

未共轭

13B148

CDC2(CDK1, 细胞周期蛋白依赖性激酶 1)

小鼠

单克隆抗体

抗体

100微升

473美元

13B148

IgG1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration. Applications: Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

CDC2;CDC28A;P34CDC2;MGC111195;DKFZp686L20222;CDK1

Purified recombinant fragment of CDC2 expressed in E. coli.

人类

腹水

Ascites fluid, 0.03% sodium azide.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 13B148 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of CDC2 expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫组化免疫印迹

-20°C

1. Mol Biol Cell. 2008 Aug;19(8):3536-43. 2. Eur J Cancer. 2009 May;45(8):1466-73.