抗体

CDKN2A, CT (Cyclin-dependent Kinase Inhibitor 2A, Isoforms 1/2/3, Cyclin-dependent Kinase 4 Inhibitor A, CDK4I, Multiple Tumor Suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A, CDKN2, MTS1)

030085

100微升

502美元

单克隆

小鼠

未共轭

13B163

CDKN2A, CT (Cyclin-dependent Kinase Inhibitor 2A, Isoforms 1/2/3, Cyclin-dependent Kinase 4 Inhibitor A, CDK4I, Multiple Tumor Suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A, CDKN2, MTS1)

小鼠

单克隆抗体

抗体

100微升

502美元

13B163

IgG1

CDKN2A acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. Applications: Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Flow Cytometry: 1:200-1:400 ELISA: 1:10000 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

纯化重组片段对应于人CDKN2A(AA: 1-156)在大肠杆菌中表达

人类

腹水

Ascites fluid containing 0.03% sodium azide.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 13B163 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid containing 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human CDKN2A (AA: 1-156) expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC免疫组化免疫印迹

-20°C

1.Clin Cancer Res. 2011 Dec 1;17(23):7413-23. 2.Appl Immunohistochem Mol Morphol. 2011 Dec;19(6):562-8.