抗体

FAK(FAK1, 粘着激酶1, PTK2蛋白酪氨酸激酶2)

030211

100微升

473美元

单克隆

小鼠

未共轭

13B289

FAK(FAK1, 粘着激酶1, PTK2蛋白酪氨酸激酶2)

小鼠

单克隆抗体

抗体

100微升

473美元

13B289

IgG1

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.RD: Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a “Focal Adhesion Targeting” (FAT) sequence, a 125 amino acid sequence at the C-terminus. FAK plays an important role in migration, cell spreading, differentiation, cytoskeleton protein phosphorylation, apoptosis and acceleration of the G1 to S phase transition of the cell cycle. It associates with several different signaling proteins such as Src-family PTKs, p130Cas, Shc, Grb2, PI 3-kinase, and paxillin. This enables FAK to function within a network of integrin-stimulated signaling pathways leading to the activation of targets such as the ERK and JNK/mitogen-activated protein kinase pathways. FAK is also linked to oncogenes at biochemical and functional levels. Increased expression and/or activity of FAK in various tumors has been correlated with enhanced migration and invasiveness of human tumor cells in addition to promoting increased cell proliferation. Applications: Western Bloting: 1:500-1:2000. Immunofluorescence: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

FAK;FADK;FAK1;FRNK;pp125FAK;PTK2

纯化重组片段对应于人FAK在大肠杆菌中表达

人类

腹水

Ascites fluid, 0.03% sodium azide.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 13B289 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human FAK expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC免疫印迹

-20°C

1. J Biol Chem. 2009 Aug 21;284(34):22865-77. 2. Biochem Biophys Res Commun. 2009 Oct 16;388(2):301-5.