抗体

IHOG (Interference hedgehog)  (Drosophila)

030326

100微升

473美元

单克隆

小鼠

未共轭

13B404

IHOG (Interference hedgehog)  (Drosophila)

小鼠

单克隆抗体

抗体

100微升

473美元

13B404

IgG1

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response. Applications: Western Bloting: 1:500-1:2000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

CG9211; CT26314; Dmel CG9211; ihog; Ihog

纯化重组片段对应于人IHOG在大肠杆菌中表达

果蝇

腹水

Ascites fluid, 0.03% sodium azide.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 13B404 Host: mouse Source: drosophila Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human IHOG expressed in E. coli. Specificity: drosophila Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫印迹

-20°C

1. Cell. 2006 Apr 21;125(2):343-57. 2. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17208-13.