抗体

IL1B

030337

100微升

502美元

单克隆

小鼠

未共轭

13B415

IL1B

小鼠

单克隆抗体

抗体

100微升

502美元

13B415

IgG1

Interleukin-1b (IL-1b), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1b is synthesized as a 31kD precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1b is a good indicator of caspase-1 activity. Applications: Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

纯化重组片段对应于人IL1B在大肠杆菌中表达

人类

腹水

Ascites fluid, 0.03% sodium azide.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 13B415 Host: mouse Source: human Concentration: Not determined Form: Ascites fluid, 0.03% sodium azide. Purity: Ascites Immunogen: Purified recombinant fragment of human IL1B expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫组化免疫印迹

-20°C

1. Dinarello, C.A. (1998) Int. Rev. Immunol. 16, 457-499. 2. Burns, K. et al. (1998) J. Biol. Chem. 273, 12203-12209. 3. Cao, Z. et al. (1996) Nature 383, 443-446. 4. Cao, Z. et al. (1996) Science 271, 1128-1131. 5. Wesche, H. et al. (1997) Immunity 7, 837-847. 6. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256. 7. Thornberry, N. A. et al. (1992) Nature 356, 768-774. 8. Cerretti, D. P. et al. (1992) Science 256, 97-100.