抗体

NEDD8

030482

100微升

502美元

单克隆

小鼠

未共轭

13B560

NEDD8

小鼠

单克隆抗体

抗体

100微升

502美元

13B560

IgG1

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Tissue specificity: Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes. Applications: Western Bloting: 1:500-1:2000. Immunohistochemistry: 1:200-1:1000. Immunofluorescence: 1:200-1:1000. Flow cytometry: 1:200-1:400. ELISA: Suggested dilution 1:10000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

NEDD-8

纯化重组片段对应于人NEDD8在大肠杆菌中表达

人类

纯化

Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer.

~1毫克/毫升

Product Type: Mab Isotype: IgG1 Clone No: 13B560 Host: mouse Source: human Concentration: ~1mg/ml Form: Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. Purity: Purified Immunogen: Purified recombinant fragment of human NEDD8 expressed in E. coli. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC免疫组化免疫印迹

-20°C

1. Cell. 2009 Jul 23;138(2):389-403. 2. Biochem Biophys Res Commun. 2009 Apr 10;381(3):443-7.