p53 (Tumor suppressor p53)
Product wo Prefix :
p53 (Tumor suppressor p53)
Size1 List :
p53 responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it s believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes th.at inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
LFS1, TRP53, TP53
Recombinant protein corresponding to a fragment of human p53, expressed in E. coli (Gene ID 7157).
Supplied as a liquid, 0.03% sodium azide.
Product Type: Mab Isotype: IgG1 Clone No: 13B597 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid, 0.03% sodium azide. Purity: Ascites Immunogen: Recombinant protein corresponding to a fragment of human p53, expressed in E. coli (Gene ID 7157). Specificity: Recognizes human p53. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
1. Cancer Invest. 2009 Jan;27(1):96-104. 2. Anticancer Res. 2007 Nov-Dec;27(6B):4143-8. 3. Mol Cell. 2008 Feb 1;29(2):217-31.
Picture 1 File Name :
4 Technology Way
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