产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
GBL (Target of rapamycin complex subunit LST8,MLST8,GBL, LST8) discontinued
目录 :
030985
规格 :
200微升
价格 :
511美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
211CT7.1。 1
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
030985
Product wo Prefix :
GBL (Target of rapamycin complex subunit LST8,MLST8,GBL, LST8) discontinued
宿主 :
小鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
200微升
Size1 List :
511美元
克隆#USB :
211CT7.1。 1
抗体亚型 :
IgG1, k
Desc1 :
Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Applications: Suitable for use in Western Blot, ELISA. Recommended Dilution: ELISA: 1:1000 Western Blot: 1:15~60 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Calc Crossreactivity :
Mo
又名 :
Target of rapamycin complex subunit LST8,MLST8,GBL, LST8
免疫原 :
GBL Mab is generated from mouse immunized with GBL recombinant protein.
特异性 :
小鼠
纯度 :
Purified by Protein G affinity chromatography.
形式 :
以溶解在PBS液体方式提供, pH值7.2, 0.09% 叠氮化钠
浓度 :
As reported
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: 211CT7.1.1 Host: mouse Source: mouse Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: GBL Mab is generated from mouse immunized with GBL recombinant protein. Specificity: Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E免疫印迹
储存温度 :
-20°C
参考文献 :
Ali, S.M., et al. J. Biol. Chem. 280(20):19445-19448(2005) Inoki, K., et al. Microbiol. Mol. Biol. Rev. 69(1):79-100(2005) Sarbassov, D.D., et al. Science 307(5712):1098-1101(2005) Jacinto, E., et al. Nat. Cell Biol. 6(11):1122-1128(2004) Kim, D.H., et al. Mol. Cell 11(4):895-904(2003)
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。