抗体

肌动蛋白, alpha1, CT(ACTA1, ACTA, 肌动蛋白, 甲骨骼肌, 阿尔法肌动蛋白)

031572

200微升

569美元

多克隆

domestic rabbit

未共轭

肌动蛋白, alpha1, CT(ACTA1, ACTA, 肌动蛋白, 甲骨骼肌, 阿尔法肌动蛋白)

兔

多克隆抗体

抗体

200微升

569美元

IgG

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence: 1:10-1:50 Flow Cytometry: 1:10-1:50 Immunohistochemistry (FFPE): 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

ACTA1, ACTA, 肌动蛋白, 甲骨骼肌, 阿尔法肌动蛋白

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the C-terminal region of human Actin, alpha1. Species Sequence Homology: bovine, chicken, mouse, porcine, rat, rabbit and Xenopus

识别人类肌动蛋白, alpha1

Purified by Protein A and peptide affinity chromatography.

以溶解在PBS液体方式提供, pH值7.2, 0.09% 叠氮化钠

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the C-terminal region of human Actin, alpha1. Species Sequence Homology: bovine, chicken, mouse, porcine, rat, rabbit and Xenopus Specificity: Recognizes human Actin, alpha1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF免疫组化免疫印迹

-20°C

1. Kim, E.Y., et al. Am. J. Physiol. Renal Physiol. 299 (3), F594-F604 (2010). 2. Haigh, S.E., et al. Neuromuscul. Disord. 20(6):363-374(2010). 3. Yu, G., et al. J Clin Neurosci 7(6):766-769(2010). 4. Yu, C.H., et al. PLoS ONE 5 (7), E11878 (2010). 5. Licastro, F., et al. Curr. Pharm. Des. 16(7):783-788(2010)

https://usbio-images.r.worldssl.net/prodimages/34/031572_1.jpg

Confocal immunofluorescent analysis of A549 cells using 031572 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

https://usbio-images.r.worldssl.net/prodimages/34/031572_5.jpg

Flow Cytometric analysis of CEM cells using 031572 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.