抗体

ATG3, CT (ATG3, APG3, APG3L, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96)

032187

200微升

569美元

多克隆

domestic rabbit

未共轭

ATG3, CT (ATG3, APG3, APG3L, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96)

兔

多克隆抗体

抗体

200微升

569美元

IgG

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy. Applications: Suitable for use in Western Blot, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

ATG3, APG3, APG3L, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96

ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90~120 amino acids from the C-term of human APG3L.

人类

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

以溶解在PBS液体方式提供, pH值7.2, 0.09% 叠氮化钠

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS. Immunogen: ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90~120 amino acids from the C-term of human APG3L. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E免疫印迹

-20°C

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)

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