抗体

KRAS, CT (KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed) (APC)

037592-APC

200微升

711美元

多克隆

domestic rabbit

APC

KRAS, CT (KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed) (APC)

兔

多克隆抗体

APC

抗体

200微升

711美元

IgG

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. Applications: Suitable for use in Western Blot, Immunofluorescence, Flow Cytometry and FLISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Hu Mo Rt

KRAS, KRAS2, RASK2, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 146-174 near the C-terminal region of human KRAS.

Recognizes human KRAS. Species Crossreactivity: mouse, rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

As Reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 146-174 in the C-terminal region of human KRAS. Specificity: Recognizes human KRAS. Species Crossreactivity: mouse, rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC FL IF免疫印迹

4°C不冻结

1. Bruckman, K.C., et al. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 110(5):632-637(2010). 2. Wong, K.K., et al. Am. J. Pathol. 177(4):1611-1617(2010). 3. Irahara, N., et al. Diagn. Mol. Pathol. 19(3):157-163(2010). 4. Carotenuto, P., et al. Pharmacogenomics 11(8):1169-1179(2010). 5. Leventopoulos, G., et al. Clin. Exp. Rheumatol. 28(4):556-557(2010)

https://usbio-images.r.worldssl.net/prodimages/37/037592-APC_1.jpg

Confocal immunofluorescent analysis of WiDr cells using 037592 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

https://usbio-images.r.worldssl.net/prodimages/37/037592-APC_5.jpg

Flow Cytometric analysis of Hela cells using 037592 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.