抗体

NCL, ID (E443) (NCL, Nucleolin, Protein C23)

038833

200微升

569美元

多克隆

domestic rabbit

未共轭

NCL, ID (E443) (NCL, Nucleolin, Protein C23)

兔

多克隆抗体

抗体

200微升

569美元

IgG

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Immunofluorescence: 1:10-50 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

NCL, Nucleolin, Protein C23

NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of human NCL.

人类

Purified by Protein A affinity chromatography.

以溶解在PBS液体方式提供, pH值7.2, 0.09% 叠氮化钠

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by Protein A affinity chromatography. Immunogen: NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of human NCL. Specificity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF免疫组化免疫印迹

-20°C

Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010) Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010) Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010) Bertrand, L., et al. J. Virol. 84(1):109-118(2010) Jerke, U., et al. PLoS ONE 4 (12), E8302 (2009) :

https://usbio-images.r.worldssl.net/prodimages/3/038833_1.jpg

Fluorescent confocal image of Hela cell stained with NCL Antibody (Center E443). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.