产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Wilms瘤抑癌基因, 号码(E361)(Wilms瘤抑癌基因, 肾母细胞瘤蛋白, WT33)(APC)
目录 :
043900-APC
规格 :
200微升
价格 :
711美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
APC
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
043900-APC
Product wo Prefix :
Wilms瘤抑癌基因, 号码(E361)(Wilms瘤抑癌基因, 肾母细胞瘤蛋白, WT33)(APC)
宿主 :
产品类型 :
多克隆抗体
共轭标签 :
APC
类别 :
抗体
尺寸1 :
200微升
Size1 List :
711美元
抗体亚型 :
IgG
Desc1 :
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and FLISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Formalin fixed and paraffin embedded tissues Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Calc Crossreactivity :
Hu Mo
又名 :
Wilms瘤抑癌基因, 肾母细胞瘤蛋白, WT33
免疫原 :
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the central region of human WT1. Species Sequence Homology: rat, porcine, Xenopus
特异性 :
Recogizes human WT1. Species Crossreactivity: mouse
纯度 :
Purified by Protein A and peptide affinity chromatography.
形式 :
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
浓度 :
As Reported
Desc2 :
Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As Reported Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 346-375 in the central region of human WT1. Species Sequence Homology: rat, porcine, Xenopus Specificity: Recogizes human WT1. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC FL IF免疫组化免疫印迹
储存温度 :
4°C不冻结
参考文献 :
Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010) Dohi, S., et al. Anticancer Res. 30(8):3187-3192(2010) Rocquain, J., et al. BMC Cancer 10, 401 (2010) : Wagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) : Mitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/36/043900-APC_1.jpg
Picture4文本 :
Confocal immunofluorescent analysis with MCF-7 cell using 043900 and Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nucleus (blue).
Picture 5 File Name :
https://usbio-images.r.worldssl.net/prodimages/36/043900-APC_5.jpg
Picture5文本 :
Flow cytometric analysis of MCF-7 cells using 043900 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
更多信息或购买 :
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。