抗体

CD74 (HLA Class II Histocompatibility Antigen gamma Chain, HLA-DR Antigens-associated Invariant Chain, Ia Antigen-associated Invariant Chain)

138525

20微克

353美元

单克隆

小鼠

未共轭

LN-2

CD74 (HLA Class II Histocompatibility Antigen gamma Chain, HLA-DR Antigens-associated Invariant Chain, Ia Antigen-associated Invariant Chain)

小鼠

单克隆抗体

抗体

20微克

353美元

LN-2

IgG1, k

CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Daudi Cells. Tonsil or lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

SU-DHL淋巴瘤细胞

Recognizes human CD74. Species Crossreactivity: mouse. Can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138525-AF.

~0.2毫克/毫升

Product Type: Mab Isotype: IgG1,k Clone No: LN-2 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138525-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: SU-DHL-4 lymphoma cells Specificity: Recognizes human CD74. Species Crossreactivity: mouse. Can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF免疫组化免疫沉淀免疫印迹

-20°C

1. Leng et.al JEM 197 (11); 1467-1476 (June 2003) 2. Becker-Herman et.al MBC 16(11) 5061-5069 (November 2005) 3. Diana et.al Blood 107(12) 4807-4816 (15 June 2006)

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