产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
Cytokeratin, pan (Pan Keratin)
目录 :
138538
规格 :
20微克
价格 :
353美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
AE-1/AE-3
反应物种 :
人类
更多信息或购买 :
US Biological 产品网页
文章摘录数: 1
参考文献
Wang X, Deavers M, Patenia R, Bassett R, Mueller P, Ma Q, et al. Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease. J Transl Med. 2006;4:30 pubmed
产品信息
目录号 :
138538
Product wo Prefix :
Cytokeratin, pan (Pan Keratin)
宿主 :
小鼠
产品类型 :
单克隆抗体
类别 :
抗体
尺寸1 :
20微克
Size1 List :
353美元
克隆#USB :
AE-1/AE-3
抗体亚型 :
IgG1, k
Desc1 :
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5 , CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing ce lls in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml for 2 hours at RT Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissues sections in 10mM sodium citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: Skin, Adeno- or Squamous carcinomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Bo Ca Ch Hu Mk Mo Rb Rt
免疫原 :
Human epidermal keratin
特异性 :
Recognizes human pan cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken.
纯度 :
Purified by Protein G affinity chromatography.
形式 :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA & azide, see 138538-AF.
浓度 :
~0.2毫克/毫升
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: AE-1/AE-3 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA & azide, see 138538-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Human epidermal keratin Specificity: Recognizes human pan cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF免疫组化免疫印迹
储存温度 :
-20°C
参考文献 :
1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. 2. Tseng SCG et. al. Cell 1982; 30361.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/22/138538_1.jpg
更多信息或购买 :
US Biological 产品网页
公司信息
US Biological
4 Technology Way
Salem, MA01970
tech@usbio.net
https://www.usbio.net
800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌，致力于用真实、有价值的个性化采购经验降低研究用成本。