抗体

Keratin 18 (Cytokeratin 18, CK18, Keratin, Type I Cytoskeletal 18)

138558

20微克

353美元

单克隆

小鼠

未共轭

DA7

Keratin 18 (Cytokeratin 18, CK18, Keratin, Type I Cytoskeletal 18)

小鼠

单克隆抗体

抗体

20微克

353美元

DA7

IgG1, k

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: MCF-7, HeLa cells, Breast Cancer Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Human breast cancer PMC 42 cells

Recognizes human keratin 18. Does not react with mouse, rat, hamster, bovine, canine, porcine and sheep.

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138558-AF.

~0.2毫克/毫升

Product Type: Mab Isotype: IgG1,k Clone No: DA7 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138558AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Human breast cancer PMC 42 cells Specificity: Recognizes human keratin 18. Does not react with mouse, rat, hamster, bovine, canine, porcine and sheep. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF免疫组化免疫沉淀免疫印迹

-20°C

1. Lane EB et. al. Sem Cancer Biol 1:165-179 (1990). 2. Lauerova et. al. Hybridoma 7:495-504 (1988).

https://usbio-images.r.worldssl.net/prodimages/32/138558_1jpeg